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Caspase-10 Activity Assay Kit

For research use only. Not intended for any clinical use.
Cat.No.
PTAK-011
Description
The Caspase-10 Activity Assay Kit (Fluorometric) is used for detecting the activity of caspases that recognize the sequence AEVD using fluorometric method. The assay is based on detection of cleavage of substrate AEVD-AFC (AFC:7-amino-4- trifluoromethyl coumarin).
Applications
Useful for assaying the activity of caspase-10
Caution
Avoid freeze/thaw cycles
Component
•active caspase-10
•caspase-10 assay buffer
•caspase-10 substrate (AEVD-AFC)
•DTT
•caspase-10 inhibitor (Z-VAD-FMK)
•Microtiter plate
Method
Fluorescence
Size/Form
100 assays

Publications

Q & A

Customer Reviews

Customer Q&As
What is Caspase-10?

A: Caspase-10 is an enzyme that, in humans, is encoded by the CASP10 gene. It plays a crucial role in programmed cell death, or apoptosis.

What is the role of caspase-10 in apoptosis?

A: Caspase-10 functions as an initiator caspase within the extrinsic apoptosis pathway. Activated caspase-10 cleaves and activates downstream effector caspases, such as caspase-3, -6, and -7, leading to the execution of apoptosis.

What diseases are caspase-10 associated with?

A: Mutations or dysregulation of caspase-10 can contribute to various diseases, including autoimmune disorders, immunodeficiencies, and cancer.

What is the role of caspase-10 in cancer?

A: Alterations in caspase-10 expression levels or activity can promote tumor growth and resistance to apoptosis, contributing to cancer progression.

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