Caspase-1 Activity Assay Kit

Name: Caspase-1 Activity Assay Kit
SKU: PTAK-002

For research use only. Not intended for any clinical use.

Cat.No.

PTAK-002

Description

The Caspase-1 Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on detection of cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin).

Applications

Useful for assaying the activity of caspase-1

Caution

Avoid freeze/thaw cycles

Component

•Caspase-1
•Caspase-1 assay buffer
•Caspase-1 substrate (YVAD-AFC)
•DTT
•Caspase-1 inhibitor (Z-VAD-FMK)
•Microtiter plate

Method

Fluorescence

Size/Form

100 assays

Publications

Q & A

Customer Reviews

Customer Q&As

What is caspase-1?

A: Caspase-1 is a protein enzyme that is a member of the caspase family of enzymes, which are responsible for initiating and executing programmed cell death (apoptosis).

What is the role of caspase-1 in inflammatory response?

A: Caspase-1 is involved in the maturation and secretion of proinflammatory cytokines, such as interleukin-1β (IL-1β) and interleukin-18 (IL-18). It cleaves these proinflammatory cytokines to their active forms, which then trigger the inflammatory response to combat infections and promote healing.

Where is caspase-1 primarily expressed?

A: Due to its role in inflammatory immune responses, it is highly expressed in immune organs such as liver, kidney, spleen, and blood (neutrophils).

What is the structure of caspase-1?

A: Caspase-1 is produced as a zymogen that can then be cleaved into 20 kDa (p20) and 10 kDa (p10) subunits that become part of the active enzyme.

What diseases are caspase-1 associated with?

A: Caspase-1 is associated with several inflammatory diseases, and its dysregulation can contribute to chronic inflammation and autoimmune conditions.

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