PfoI

Name: PfoI
SKU: EROT0435

For research use only. Not intended for any clinical use.

Cat.No.

EROT0435

Reaction Buffer

33mM Tris-acetate (pH7.9 at 37 °C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA

Reaction Conditions

Incubate at 37 °C

Recognition Site

T↓CCNGGA

Size/Form

200 Units

Storage

10mM Tris-HCl (pH 7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Publications

Q & A

Customer Reviews

Customer Q&As

Unexpected DNA bands were observed on agarose gel electrophoresis after restriction digestion. What may have caused this?

A: Unexpected cleavage patterns may be caused by the following reasons:Star activity of the restriction enzyme,Partial or incomplete cleavage (incomplete restriction reaction),ontamination with non-specific endonucleases,Improper reaction setup.

Can I double digest my DNA using a conventional restriction enzyme and a restriction enzyme?

A: For optimal results with fast reaction and 100% buffer compatibility, we recommend using restriction enzymes in double digestion.

What is the recognition and cutting sequence of PfoI enzyme?

A: PfoI enzyme recognizes the sequence T^CCNGGA and cuts best at 37°C in Tango buffer?.

Under what reaction conditions is the activity of PfoI enzyme optimal?

A: PfoI enzyme exhibits optimal activity at 37°C in Tango buffer.

What is the sensitivity of PfoI enzyme to DNA methylation?

A: The cleavage activity of PfoI enzyme is impaired by overlapping dam methylation and blocked by overlapping dcm methylation. To avoid dam or dcm methylation, a dam-, dcm- strain such as GM2163 can be used.

Is PfoI enzyme able to withstand heat inactivation, and if so, what temperature and duration are required?

A: PfoI enzyme can be denatured by heating at 65°C for 20 minutes?.

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