Mouse Nr1h3 lentiviral particles

Name: Mouse Nr1h3 lentiviral particles
SKU: LV00402Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV00402Z

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV00402Z
Description	Mouse Nr1h3 ORF cDNA is cloned into the lentiviral expression vector for maximal stable delivery. The ready-to-use lentiviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts.
Product Type	Lentiviral particle
Gene	Nr1h3
Species	Mouse
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	Nr1h3 nuclear receptor subfamily 1, group H, member 3 [ Mus musculus ]
Gene Symbol	Nr1h3
Synonyms	NR1H3; nuclear receptor subfamily 1, group H, member 3; oxysterols receptor LXR-alpha; LXR alpha; liver X receptor alpha; ubiquitously-expressed nuclear receptor 1; LXR; RLD1; Unr1; AU018371;
GeneID	22259
mRNA Refseq	NM_001177730
Protein Refseq	NP_001171201
Function	DNA binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; protein binding; protein heterodimerization activity; receptor activity; retinoid X receptor binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; sterol response element binding; contributes_to transcription regulatory region DNA binding; transcription regulatory region DNA binding; zinc ion binding;
Pathway	Adipogenesis, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem; Nuclear Receptors, organism-specific biosystem;

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