Human NR1H3 KO Cell Line-Hela

Name: Human NR1H3 KO Cell Line-Hela
SKU: CSC-RT2842
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2842

Target Gene : NR1H3 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2842
Description	This cell is a stable cell line with a homozygous knockout of human NR1H3 using CRISPR/Cas9.
Target Gene	NR1H3
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NR1H3 nuclear receptor subfamily 1, group H, member 3 [ Homo sapiens ]
Gene Symbol	NR1H3
Synonyms	LXRA; LXR-a; RLD-1
Gene Description	nuclear receptor subfamily 1, group H, member 3
GeneID	10062
Uni ProtID	Q8IW13
mRNA Refseq	NM_001130102.2
Protein Refseq	NP_001123574.1
Chromosome Location	11p11.2
Function	DNA binding; cholesterol binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; protein binding; protein heterodimerization activity; retinoid X receptor binding; sequence-specific DNA binding; steroid hormone receptor activity; sterol response element binding; transcription coactivator activity; transcription regulatory region DNA binding; contributes_to transcription regulatory region DNA binding; zinc ion binding;
Pathway	Adipogenesis, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem; Nuclear Receptors, organism-specific biosystem;
MIM	602423

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