Monkey TNFRSF9 Stable Cell Line - CHO-K1

Name: Monkey TNFRSF9 Stable Cell Line - CHO-K1
SKU: CSC-RO0190
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0190

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0190
Description	This cell line is engineered to stably overexpress the monkey TNFRSF9 in CHO-K1 cells.
Introduction	This cell line is constructed by transfection of monkey TNF receptor superfamily member 9 (TNFRSF9) into CHO-K1, followed by stable cell selection. The expression of TNFRSF9 has been analyzed through flow cytometry.
Target Gene	TNFRSF9
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

What are the regulatory requirements for the use of the Monkey TNFRSF9 Stable Cell Line in clinical trials?

A: Regulatory requirements vary by country and include ensuring the cell line's quality, safety, and efficacy, as well as compliance with Good Manufacturing Practices (GMP) for any clinical applications.

What are the potential applications of the Monkey TNFRSF9 Stable Cell Line in drug discovery and development?

A: The cell line can be used to screen for compounds that modulate the 4-1BB receptor, aiding in the discovery of new drugs for cancer and autoimmune diseases where the 4-1BB pathway is implicated.

What is the origin of the Monkey TNFRSF9 Stable Cell Line - CHO-K1?

A: The Monkey TNFRSF9 Stable Cell Line - CHO-K1 originates from the Chinese Hamster Ovary (CHO) cell line, specifically a subclone known as CHO-K1, established in 1957 by Theodore Puck from adult Chinese hamster ovary biopsies.

What genetic modifications have been made to create the TNFRSF9 Stable Cell Line?

A: The CHO-K1 cell line has been genetically engineered to stably express the human TNFRSF9 gene, which encodes the 4-1BB receptor, enabling the cell line to model the function and regulation of the 4-1BB receptor in various biological processes.

How is the Monkey TNFRSF9 Stable Cell Line utilized in immunotherapy research?

A: The cell line is employed to investigate the role of the 4-1BB receptor in immune cell activation, particularly in T cell responses, aiding in the development of therapeutic strategies targeting the 4-1BB pathway for cancer and autoimmune diseases.

How is the Monkey TNFRSF9 Stable Cell Line preserved and revived for future use?

A: The cell line is typically cryopreserved in liquid nitrogen using a freezing medium containing dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS). For revival, cells are thawed rapidly and transferred to a culture medium for recovery and expansion.

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