Cat.No. : CSC-RO0094 Host Cell: HEK293T
| Cat. No. | CSC-RO0094 |
| Description | HEK293T-TNFSF14 cell line is a stably transfected cell line which expresses human TNF superfamily member 14 (TNFSF14). |
| Gene | TNFSF14 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Human TNFSF14 Stable Cell Line-HEK293T originates from the HEK293T (Human Embryonic Kidney 293T) cell line and has been engineered to stably express the human TNFSF14 (Tumor Necrosis Factor Superfamily Member 14) gene. Typically, this cell line is established by introducing the TNFSF14 gene construct into HEK293T cells, followed by selection and maintenance of stable expression.
A: The genotype of Human TNFSF14 Stable Cell Line-HEK293T includes the genetic makeup of HEK293T cells, along with stable expression of the human TNFSF14 gene. In terms of phenotype, these cells express the TNFSF14 protein, which is a cell membrane receptor typically associated with immune regulation and signaling.
A: Human TNFSF14 Stable Cell Line-HEK293T has several applications, including studying the interaction of TNFSF14 with its ligands, investigating immune checkpoint pathways, evaluating potential immunotherapies, and screening molecules that modulate TNFSF14 signaling. It is valuable for research related to immune regulation and the development of immunotherapies.
A: Yes, the use of Human TNFSF14 Stable Cell Line-HEK293T is subject to regulatory and ethical guidelines, especially when working with human-derived cell lines in research. Researchers are expected to adhere to relevant ethical standards, obtain necessary approvals, and ensure compliance with applicable regulations to ensure ethical compliance of experiments.
A: Human TNFSF14 Stable Cell Line-HEK293T should be stored in liquid nitrogen for long-term preservation. To revive these cells, they can be thawed rapidly at 37 degrees Celsius and seeded into culture dishes using appropriate growth medium and conditions.
A: When working with Human TNFSF14 Stable Cell Line-HEK293T, researchers should follow general biosafety guidelines for handling human-derived cell lines. This includes working in appropriate biosafety cabinets, using personal protective equipment, and following established laboratory safety protocols to prevent contamination and ensure safe handling.
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