Human TNFSF14 Stable Cell Line-HEK293T

Name: Human TNFSF14 Stable Cell Line-HEK293T
SKU: CSC-RO0094
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0094

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0094
Description	HEK293T-TNFSF14 cell line is a stably transfected cell line which expresses human TNF superfamily member 14 (TNFSF14).
Target Gene	TNFSF14
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFSF14 tumor necrosis factor (ligand) superfamily, member 14 [ Homo sapiens ]
Gene Symbol	TNFSF14
Synonyms	TNFSF14; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; CD258; HVEM L; LIGHT; LTg; delta transmembrane LIGHT; herpesvirus entry mediator A; herpesvirus entry mediator ligand; herpesvirus entry mediator-ligand; herpes virus entry mediator ligand; ligand for herpesvirus entry mediator; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT; TR2; HVEML;
Gene ID	8740
Uni Prot ID	O43557
m RNA Refseq	BC018058
Chromosome Location	19p13.3
Function	cysteine-type endopeptidase inhibitor activity involved in apoptotic process; cytokine activity; protein binding; receptor binding; tumor necrosis factor receptor binding;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem;
MIM	604520

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Customer Q&As

What is the origin of Human TNFSF14 Stable Cell Line-HEK293T, and how was it established?

A: Human TNFSF14 Stable Cell Line-HEK293T originates from the HEK293T (Human Embryonic Kidney 293T) cell line and has been engineered to stably express the human TNFSF14 (Tumor Necrosis Factor Superfamily Member 14) gene. Typically, this cell line is established by introducing the TNFSF14 gene construct into HEK293T cells, followed by selection and maintenance of stable expression.

What is the genotype and phenotype of Human TNFSF14 Stable Cell Line-HEK293T?

A: The genotype of Human TNFSF14 Stable Cell Line-HEK293T includes the genetic makeup of HEK293T cells, along with stable expression of the human TNFSF14 gene. In terms of phenotype, these cells express the TNFSF14 protein, which is a cell membrane receptor typically associated with immune regulation and signaling.

What are the primary applications of Human TNFSF14 Stable Cell Line-HEK293T in scientific research and drug development?

A: Human TNFSF14 Stable Cell Line-HEK293T has several applications, including studying the interaction of TNFSF14 with its ligands, investigating immune checkpoint pathways, evaluating potential immunotherapies, and screening molecules that modulate TNFSF14 signaling. It is valuable for research related to immune regulation and the development of immunotherapies.

Is the use of Human TNFSF14 Stable Cell Line-HEK293T subject to specific regulatory and ethical restrictions?

A: Yes, the use of Human TNFSF14 Stable Cell Line-HEK293T is subject to regulatory and ethical guidelines, especially when working with human-derived cell lines in research. Researchers are expected to adhere to relevant ethical standards, obtain necessary approvals, and ensure compliance with applicable regulations to ensure ethical compliance of experiments.

What are the recommended procedures for the storage and revival of Human TNFSF14 Stable Cell Line-HEK293T?

A: Human TNFSF14 Stable Cell Line-HEK293T should be stored in liquid nitrogen for long-term preservation. To revive these cells, they can be thawed rapidly at 37 degrees Celsius and seeded into culture dishes using appropriate growth medium and conditions.

Are there any specific biosafety precautions to be taken when handling Human TNFSF14 Stable Cell Line-HEK293T?

A: When working with Human TNFSF14 Stable Cell Line-HEK293T, researchers should follow general biosafety guidelines for handling human-derived cell lines. This includes working in appropriate biosafety cabinets, using personal protective equipment, and following established laboratory safety protocols to prevent contamination and ensure safe handling.

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