Human SIRPG Stable Cell Line-CHO-K1

Name: Human SIRPG Stable Cell Line-CHO-K1
SKU: CSC-RO0052
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0052

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0052
Description	This cell line is a stably transfected cell line which expresses Human SIRPG without any tag.
Background	The protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes.
Target Gene	SIRPG
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SIRPG signal-regulatory protein gamma [ Homo sapiens ]
Gene Symbol	SIRPG
Synonyms	CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
Gene Description	signal-regulatory protein gamma
Gene ID	55423
Uni Prot ID	Q9P1W8
m RNA Refseq	NM_080816.2
Protein Refseq	NP_543006.2
Chromosome Location	20p13
Function	protein binding;
Pathway	Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Hemostasis, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem; Signal regulatory protein (SIRP) family interactions, organism-specific biosystem;
MIM	605466

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