Human F2RL3 Stable Cell Line-CHO-K1

Name: Human F2RL3 Stable Cell Line-CHO-K1
SKU: CSC-RG0922
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0922

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0922
Background	Protease-activated receptor-4 (PAR4) belongs to a four-member family of GPCRs that are activated by proteases (see Ossovskaya et al for review). These proteases cleave a specific extracellular amino-terminal domain of the receptor to reveal a tethered ligand, which in turn activates the receptor which couples via Gq to release intracellular calcium (Nystedt, S. et al., 1995). PAR4 is expressed in the vasculature, GI tract, leukocytes, lung, and heart. PAR4 is a relevant target for coronary thromboses, inflammatory bowel disease and cancer.
Target Gene	F2RL3
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuF2RL3
Alias	F2RL3, PAR4, PAR-4
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	F2RL3 coagulation factor II (thrombin) receptor-like 3 [ Homo sapiens ]
Gene Symbol	F2RL3
Synonyms	PAR4
Gene Description	coagulation factor II (thrombin) receptor-like 3
Gene ID	9002
Uni Prot ID	Q96RI0
m RNA Refseq	NM_003950.2
Protein Refseq	NP_003941.2
Chromosome Location	19p12
Function	thrombin receptor activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Gastrin-CREB signalling pathway via PKC and MAPK, organism-specific biosystem; Hemostasis, organism-specific biosystem;
MIM	602779

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