Hsp92II

Name: Hsp92II
SKU: EROT0376

For research use only. Not intended for any clinical use.

Cat.No.

EROT0376

Description

Haemophilus influenzae 92.

Concentration

10000 units/ml

Reaction Conditions

Buffer K. 37 °C.

Recognition Site

CATG↓

Size/Form

1000 units

Storage

10mM Tris-HCl (pH 7.3), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Publications

Q & A

Customer Reviews

Customer Q&As

What is the recognition sequence for Hsp92II?

A: The recognition sequence for Hsp92II is CATG↑. The arrow indicates the cutting site.

In which buffer does Hsp92II show optimal activity?

A: Hsp92II shows optimal activity in Buffer 3.1 at 37°C. (Note: This answer is an example; the actual buffer may vary based on the manufacturer's recommendation.)

Can Hsp92II digest methylated DNA?

A: Hsp92II cannot cleave DNA if one of the adenines in its recognition site is methylated.

At what temperature should Hsp92II be inactivated or heat-killed?

A: Hsp92II can be inactivated by incubating at 65°C for 20 minutes.

Is Hsp92II compatible with other restriction enzymes for double digestion?

A: Yes, Hsp92II is compatible with several other restriction enzymes. For specific pairings, refer to the enzyme's compatibility chart provided by the manufacturer.

How long does it take for Hsp92II to achieve complete digestion?

A: Under optimal conditions, 1 μg of lambda DNA can be completely digested by 10 units of Hsp92II in 1 hour at 37°C.

Are there any known star activity or non-specific cleavage issues associated with Hsp92II?

A: Star activity is observed with Hsp92II under non-optimal conditions such as prolonged incubation or in the presence of excessive enzyme. It's recommended to follow the manufacturer's guidelines to avoid star activity.

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