scFv(Control)-CD28-41BB-CD3zeta CAR-T Lentivirus

scFv(Control)-CD28-41BB-CD3ζ CAR-T Lentivirus is a matched control reagent for CAR-T-cell research. It encodes a chimeric antigen receptor containing a control single-chain variable fragment (scFv), membrane-anchoring elements, tandem intracellular costimulatory domains from CD28 and 4-1BB (CD137), and the CD3ζ activation domain. The control scFv is selected to lack relevant recognition of the target antigen used in the experimental system, while retaining an overall receptor architecture comparable to that of an antigen-specific third-generation CAR. This design helps separate effects caused by target recognition from those related to lentiviral transduction, CAR expression, or intracellular signaling components. Lentiviral delivery supports efficient gene transfer and stable receptor expression in transduced T cells, providing a consistent control population for side-by-side experimental comparisons.

This product is intended for use as a negative control in studies of antigen-specific CAR-T-cell activity. T cells transduced with the control lentivirus can be compared with target-specific CAR-T cells when measuring transduction efficiency, receptor expression, cell viability, proliferation, differentiation, basal activation, and expansion. In co-culture assays, the control cells help establish background levels of target-cell killing, cytokine secretion, degranulation, and nonspecific responses to antigen-positive or antigen-negative cells. The construct is also useful for evaluating tonic signaling, activation caused by receptor clustering, and cellular changes associated with prolonged CAR expression. In animal studies, an appropriately validated control group may help distinguish antigen-dependent effects from responses caused by cell administration, vector exposure, culture conditions, or the tumor model itself. Because the intracellular architecture includes both CD28 and 4-1BB, the reagent is especially suitable as a backbone-matched control for third-generation CAR constructs containing the same signaling domains. Its lentiviral format supports reproducible control-cell preparation across screening, mechanistic, and preclinical research workflows.