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Cat.No. : CSC-RO0502 Host Cell: CHO-K1
| Cat. No. | CSC-RO0502 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Rat Fcgr2b. |
| Gene | Fcgr2b |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Try optimizing the culture medium formulation, adding appropriate growth factors, adjusting CO₂ concentration and temperature, and ensuring the stability of the cell culture environment.
A: Use high affinity and specificity antibodies against Fcgr2b, optimize antibody dilution ratio and incubation time, and ensure cells are well-maintained during processing and analysis.
A: Optimize cell lysis conditions, use appropriate immunoprecipitation reagents and antibodies for Fcgr2b protein, ensure sufficient incubation time, and appropriate washing steps.
A: Experiment with different transfection methods and reagents, such as electroporation or liposome-mediated transfection, and optimize DNA quality and culture conditions before and after transfection.
A: Optimize protein extraction and electrophoresis conditions, ensure the use of suitable carriers and transfer conditions for Fcgr2b protein, and optimize the choice of antibodies and detection methods.
A: Use standardized apoptosis detection methods, ensure consistency in experimental conditions, including cell density and treatment agent concentration, and perform multiple repetitions.
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