Rat Fcgr2b Stable Cell Line - CHO-K1

Name: Rat Fcgr2b Stable Cell Line - CHO-K1
SKU: CSC-RO0502
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0502

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0502
Description	This cell line is derived from CHO-K1 and is engineered to stably overexpress Rat Fcgr2b.
Target Gene	Fcgr2b
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Fcgr2b Fc receptor, IgG, low affinity IIb [ Mus musculus ]
Gene Symbol	Fcgr2b
Synonyms	CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik
Gene Description	Fc receptor, IgG, low affinity IIb
Gene ID	14130
m RNA Refseq	NM_001077189.1
Protein Refseq	NP_001070657.1
Chromosome Location	1 H3; 1 92.3 cM
Function	IgG binding; protein binding; receptor signaling protein activity;
Pathway	B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Measles, organism-specific biosystem; Measles, conserved biosystem;

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How to improve cell proliferation rate in Rat Fcgr2b Stable Cell Line during cell proliferation experiments?

A: Try optimizing the culture medium formulation, adding appropriate growth factors, adjusting CO₂ concentration and temperature, and ensuring the stability of the cell culture environment.

How to enhance the detection efficiency of Fcgr2b protein in flow cytometry analysis of Rat Fcgr2b Stable Cell Line?

A: Use high affinity and specificity antibodies against Fcgr2b, optimize antibody dilution ratio and incubation time, and ensure cells are well-maintained during processing and analysis.

How to improve protein interaction detection efficiency in co-immunoprecipitation experiments with Rat Fcgr2b Stable Cell Line?

A: Optimize cell lysis conditions, use appropriate immunoprecipitation reagents and antibodies for Fcgr2b protein, ensure sufficient incubation time, and appropriate washing steps.

How to improve transfection efficiency in Rat Fcgr2b Stable Cell Line?

A: Experiment with different transfection methods and reagents, such as electroporation or liposome-mediated transfection, and optimize DNA quality and culture conditions before and after transfection.

How to enhance the stability of protein detection in Western Blot experiments with Rat Fcgr2b Stable Cell Line?

A: Optimize protein extraction and electrophoresis conditions, ensure the use of suitable carriers and transfer conditions for Fcgr2b protein, and optimize the choice of antibodies and detection methods.

How to ensure the accuracy and reproducibility of apoptosis experiments in Rat Fcgr2b Stable Cell Line?

A: Use standardized apoptosis detection methods, ensure consistency in experimental conditions, including cell density and treatment agent concentration, and perform multiple repetitions.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *