Rat CD47 Stable Cell Line - CHO-K1

Name: Rat CD47 Stable Cell Line - CHO-K1
SKU: CSC-RO0277
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0277

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0277
Description	This cell line is derived from CHO-K1 and is engineered to stably overexpress Rat CD47.
Target Gene	CD47
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Cd47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) [ Mus musculus ]
Gene Symbol	Cd47
Synonyms	IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
Gene Description	CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
GeneID	16423
Uni ProtID	Q61735
mRNA Refseq	NM_010581.3
Protein Refseq	NP_034711.1
Chromosome Location	16 B5; 16
Function	protein binding; thrombospondin receptor activity;
Pathway	Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Hemostasis, organism-specific biosystem; Signal regulatory protein (SIRP) family interactions, organism-specific biosystem;

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

During the culture of Rat CD47 Stable Cell Line - CHO-K1, how to ensure that the stable expression of CD47 protein is not affected by the number of cell passages?

A: Regular monitoring of CD47 protein expression levels, such as through Western Blot and flow cytometry, can be done to monitor the expression stability of CD47 protein in Rat CD47 Stable Cell Line - CHO-K1 across different passage numbers.

In the freezing and revival process of Rat CD47 Stable Cell Line - CHO-K1, what are the key steps to be noted to maintain the stability of the cell line?

A: Appropriate freezing medium should be used, control the rate of freezing and revival, and replace the culture medium promptly after revival to maintain the stability of Rat CD47 Stable Cell Line - CHO-K1.

When handling Rat CD47 Stable Cell Line - CHO-K1, how to maximize the preservation of the biological activity of CD47 protein?

A: By maintaining appropriate culture conditions, avoiding over-passaging, and using proper handling methods, such as avoiding harmful reagents that may affect CD47 protein activity, the biological activity of CD47 protein in Rat CD47 Stable Cell Line - CHO-K1 can be maximized.

How to evaluate the binding affinity of CD47 protein to its ligand SIRPα in Rat CD47 Stable Cell Line - CHO-K1?

A: Surface Plasmon Resonance (SPR) technology or flow cytometry binding analysis can be used to evaluate the binding affinity of CD47 protein to its ligand SIRPα in Rat CD47 Stable Cell Line - CHO-K1.

In Rat CD47 Stable Cell Line - CHO-K1, how to ensure that the glycosylation status of CD47 protein is not affected by cell culture conditions?

A: Optimizing and maintaining constant culture conditions, and regularly monitoring the glycosylation status of CD47 protein, such as through specific glycosylation antibodies or mass spectrometry analysis, can ensure the glycosylation status of CD47 protein in Rat CD47 Stable Cell Line - CHO-K1 is not affected.

When using Rat CD47 Stable Cell Line - CHO-K1 for drug screening, how to accurately evaluate the impact of drugs on the CD47 signaling pathway?

A: Establish specific cellular functional assays, such as cell adhesion assays and immune cell activation assays, and utilize signaling pathway analysis techniques like Western Blot and real-time quantitative PCR, to accurately evaluate the impact of drugs on the CD47 signaling pathway in Rat CD47 Stable Cell Line - CHO-K1.

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