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Cat.No. : CSC-RI0110-2 Host Cell: Human embryonic kidney immortal cell line (HEK293)
| Cat. No. | CSC-RI0110-2 |
| Description | This cell line is engineered to stably express human KCNN3 ion channel gene in HEK293 cells. |
| Gene | KCNN3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Human embryonic kidney immortal cell line (HEK293) |
| Host Cell Species | Homo sapiens (Human) |
| Stability | This cell line is stable at least 10 passages. |
| Product Type | Stable cell line constitutively expressing ion channel gene(s) |
| Applications | in vitro cell-based screening and discovery of ion channel drugs |
| Quality Control | 1) Ion channel activity assay 2) Mycoplasma detection |
| Size Form | One vial of frozen cells, typically >1x10^6cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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