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Human SCNN1A/SCNN1B/SCNN1G Stable Cell Line-CHO-K1

Human SCNN1A/SCNN1B/SCNN1G Stable Cell Line-CHO-K1

Cat.No. :  CSC-RI0086 Host Cell:  CHO-K1

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Cell Line Information

Cell Culture Information

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Cat. No. CSC-RI0086
Description This cell line is engineered to overexpress human SCNN1G
Background The human SCNN1A, B and G genes encode the heteromeric ENaC channel, a constitutively active, amiloride-sensitive Na+-selective ion channel. ENaC expressed in kidney and colon is a therapeutic target in hypertension, congestive heart disease and inflammatory bowel disease.
Gene SCNN1G
Gene Species Homo sapiens (Human)
Alias SCNN1G, PHA1, BESC3, ENaCg
Host Cell CHO-K1
Host Cell Species Cricetulus griseus (Chinese hamster)
Morphology Epithelial-like
Stability Validated for at least 10 passages
Application

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Channelopathies research

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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