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Human GLP1R Stable Cell Line - HEK293

Human GLP1R Stable Cell Line - HEK293

Cat.No. :  CSC-RG01846 Host Cell:  Human embryonic kidney immortal cell line (HEK293)

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Cat. No. CSC-RG01846
Description This cell line is engineered to stably overexpress human glucagon like peptide 1 receptor (GLP1R) in HEK293 cells.
Gene GLP1R
Gene Species Homo sapiens (Human)
Host Cell Human embryonic kidney immortal cell line (HEK293)
Host Cell Species Homo sapiens (Human)
Stability This cell line is stable at least 10 passages.
Product Type Stable cell line constitutively expressing GPCR gene(s)
Applications in vitro cell-based screening and discovery of GPCR agonists and antagonists
Quality Control 1) GPCR activity assay
2) Mycoplasma detection
Size Form One vial of frozen cells, typically >1x10^6cells/vial
Shipping Dry ice
Storage Liquid nitrogen
Resistance Puromycin
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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