Human CTNNB1 Knockout Cell Line-HEK293

Name: Human CTNNB1 Knockout Cell Line-HEK293
SKU: CSC-RT2805
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2805

Target Gene : CTNNB1 Host Cell : HEK293

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2805
Description	This cell is a stable cell line with a homozygous knockout of human CTNNB1 using CRISPR/Cas9.
Target Gene	CTNNB1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa [ Homo sapiens ]
Gene Symbol	CTNNB1
Synonyms	CTNNB
Gene Description	catenin (cadherin-associated protein), beta 1, 88kDa
GeneID	1499
Uni ProtID	P35222
mRNA Refseq	NM_001904.3
Protein Refseq	NP_001895.1
Chromosome Location	3p21
Function	I-SMAD binding; R-SMAD binding; SMAD binding; alpha-catenin binding; androgen receptor binding; cadherin binding; chromatin binding; double-stranded DNA binding; enzyme binding; estrogen receptor binding; ion channel binding; ionotropic glutamate receptor binding; kinase binding; nuclear hormone receptor binding; nuclear hormone receptor binding; protein C-terminus binding; protein binding; protein kinase binding; protein phosphatase binding; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity; signal transducer activity; structural molecule activity; transcription coactivator activity; transcription coactivator activity; transcription factor binding; transcription factor binding; transcription regulatory region DNA binding;
Pathway	Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adherens junctions interactions, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cell adhesion proteins, organism-specific biosystem;
MIM	116806

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