Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0070
Target Gene : CTNNB1 Host Cell : HCT116
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0070 |
| Description | HCT116-CTNNB1 (+/-) is a cell line with a knockout of mutant CTNNB1 allele in heterozygous parental cells |
| Target Gene | CTNNB1 |
| Host Cell | HCT116 |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa [ Homo sapiens ] |
| Gene Symbol | CTNNB1 |
| Synonyms | CTNNB |
| Gene Description | catenin (cadherin-associated protein), beta 1, 88kDa |
| GeneID | 1499 |
| Uni ProtID | P35222 |
| mRNA Refseq | NM_001904.3 |
| Protein Refseq | NP_001895.1 |
| Chromosome Location | 3p21 |
| Function | I-SMAD binding; R-SMAD binding; SMAD binding; alpha-catenin binding; androgen receptor binding; cadherin binding; chromatin binding; double-stranded DNA binding; enzyme binding; estrogen receptor binding; ion channel binding; ionotropic glutamate receptor binding; kinase binding; nuclear hormone receptor binding; nuclear hormone receptor binding; protein C-terminus binding; protein binding; protein kinase binding; protein phosphatase binding; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity; signal transducer activity; structural molecule activity; transcription coactivator activity; transcription coactivator activity; transcription factor binding; transcription factor binding; transcription regulatory region DNA binding; |
| Pathway | Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adherens junctions interactions, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cell adhesion proteins, organism-specific biosystem; |
| MIM | 116806 |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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The knockdown efficiency of Human CTNNB1 (+/-) Knockout Cell Line-HCT116 unparalleled. It has made my research much more streamlined and productive.
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