Pages
Products

Human CTNNB1 (+/-) Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0070

Target Gene :   CTNNB1 Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0070
Description HCT116-CTNNB1 (+/-) is a cell line with a knockout of mutant CTNNB1 allele in heterozygous parental cells
Target Gene CTNNB1
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa [ Homo sapiens ]
Gene Symbol CTNNB1
Synonyms CTNNB
Gene Description catenin (cadherin-associated protein), beta 1, 88kDa
GeneID 1499
Uni ProtID P35222
mRNA Refseq NM_001904.3
Protein Refseq NP_001895.1
Chromosome Location 3p21
Function I-SMAD binding; R-SMAD binding; SMAD binding; alpha-catenin binding; androgen receptor binding; cadherin binding; chromatin binding; double-stranded DNA binding; enzyme binding; estrogen receptor binding; ion channel binding; ionotropic glutamate receptor binding; kinase binding; nuclear hormone receptor binding; nuclear hormone receptor binding; protein C-terminus binding; protein binding; protein kinase binding; protein phosphatase binding; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity; signal transducer activity; structural molecule activity; transcription coactivator activity; transcription coactivator activity; transcription factor binding; transcription factor binding; transcription regulatory region DNA binding;
Pathway Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adherens junctions interactions, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cell adhesion proteins, organism-specific biosystem;
MIM 116806
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Customer Reviews
Impressive

I have used Human CTNNB1 (+/-) Knockout Cell Line-HCT116 for several experiments now, and I am consistently impressed with the results.

France

Must-have

Human CTNNB1 (+/-) Knockout Cell Line-HCT116 is a must-have for any researcher looking to streamline their experiments. It is very easy to use and produces great results.

United States

Favorite

I have tried other gene knockout reagents in the past, but Human CTNNB1 (+/-) Knockout Cell Line-HCT116 is my favorite. It is reliable, efficient, and very convenient and practical.

United States

Recommend

Human CTNNB1 (+/-) Knockout Cell Line-HCT116 has made my experiments much more efficient. I would recommend it to any researcher.

United States

High-quality results

I have been using Human CTNNB1 (+/-) Knockout Cell Line-HCT116 for a while now, and it consistently produces high-quality results.This is definitely trustworthy experimental data.

United States

Unparalleled

The knockdown efficiency of Human CTNNB1 (+/-) Knockout Cell Line-HCT116 unparalleled. It has made my research much more streamlined and productive.

United Kingdom

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction