Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0074
Target Gene : CTNNB1 Host Cell : HCT116
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0074 |
| Description | HCT116-CTNNB1 (-/-) is a cell line with a knockout of wild type CTNNB1 allele in heterozygous parental cells |
| Target Gene | CTNNB1 |
| Host Cell | HCT116 |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa [ Homo sapiens ] |
| Gene Symbol | CTNNB1 |
| Synonyms | CTNNB |
| Gene Description | catenin (cadherin-associated protein), beta 1, 88kDa |
| GeneID | 1499 |
| Uni ProtID | P35222 |
| mRNA Refseq | NM_001904.3 |
| Protein Refseq | NP_001895.1 |
| Chromosome Location | 3p21 |
| Function | I-SMAD binding; R-SMAD binding; SMAD binding; alpha-catenin binding; androgen receptor binding; cadherin binding; chromatin binding; double-stranded DNA binding; enzyme binding; estrogen receptor binding; ion channel binding; ionotropic glutamate receptor binding; kinase binding; nuclear hormone receptor binding; nuclear hormone receptor binding; protein C-terminus binding; protein binding; protein kinase binding; protein phosphatase binding; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity; signal transducer activity; structural molecule activity; transcription coactivator activity; transcription coactivator activity; transcription factor binding; transcription factor binding; transcription regulatory region DNA binding; |
| Pathway | Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adherens junctions interactions, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cell adhesion proteins, organism-specific biosystem; |
| MIM | 116806 |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The knockdown efficiency of Human CTNNB1 (-/-) Knockout Cell Line-HCT116 is unmatched. It is the only gene editing reagent I trust.
I would highly recommend TNNB1 (-/-) Knockout Cell Line-HCT116 to any researcher looking to streamline their gene editing process.
The efficiency of TNNB1 (-/-) Knockout Cell Line-HCT116 is impressive. It has made my research more efficient and productive.
TNNB1 (-/-) Knockout Cell Line-HCT116 has exceeded my expectations. It consistently produces high-quality results and is very convenient and useful.
I have tried other gene knockout reagents some time previously, but TNNB1 (-/-) Knockout Cell Line-HCT116 is by far the best. It is easy to use and produces reliable results.
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