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Human CTNNB1 (-/-) Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0074

Target Gene :   CTNNB1 Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0074
Description HCT116-CTNNB1 (-/-) is a cell line with a knockout of wild type CTNNB1 allele in heterozygous parental cells
Target Gene CTNNB1
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name CTNNB1 catenin (cadherin-associated protein), beta 1, 88kDa [ Homo sapiens ]
Gene Symbol CTNNB1
Synonyms CTNNB
Gene Description catenin (cadherin-associated protein), beta 1, 88kDa
GeneID 1499
Uni ProtID P35222
mRNA Refseq NM_001904.3
Protein Refseq NP_001895.1
Chromosome Location 3p21
Function I-SMAD binding; R-SMAD binding; SMAD binding; alpha-catenin binding; androgen receptor binding; cadherin binding; chromatin binding; double-stranded DNA binding; enzyme binding; estrogen receptor binding; ion channel binding; ionotropic glutamate receptor binding; kinase binding; nuclear hormone receptor binding; nuclear hormone receptor binding; protein C-terminus binding; protein binding; protein kinase binding; protein phosphatase binding; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity; signal transducer activity; structural molecule activity; transcription coactivator activity; transcription coactivator activity; transcription factor binding; transcription factor binding; transcription regulatory region DNA binding;
Pathway Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adherens junctions interactions, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cell adhesion proteins, organism-specific biosystem;
MIM 116806
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Unmatched

The knockdown efficiency of Human CTNNB1 (-/-) Knockout Cell Line-HCT116 is unmatched. It is the only gene editing reagent I trust.

Germany

Streamline process

I would highly recommend TNNB1 (-/-) Knockout Cell Line-HCT116 to any researcher looking to streamline their gene editing process.

United States

Efficient and productive

The efficiency of TNNB1 (-/-) Knockout Cell Line-HCT116 is impressive. It has made my research more efficient and productive.

United States

User-friendly

TNNB1 (-/-) Knockout Cell Line-HCT116 has exceeded my expectations. It consistently produces high-quality results and is very convenient and useful.

France

The best

I have tried other gene knockout reagents some time previously, but TNNB1 (-/-) Knockout Cell Line-HCT116 is by far the best. It is easy to use and produces reliable results.

United States

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