Pages
Products

Human CLEC7A Stable Cell Line - BEAS-2B

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01358

Host Cell :   BEAS-2B Size :   >1x106 frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO01358
Description This cell line is engineered to stably express Homo sapiens (human) C-type lectin domain containing 7A (CLEC7A) in Human immortalized bronchial epithelial cell line (BEAS-2B). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Product Type Human gene overexpression stable cell line
Target Gene CLEC7A
Gene Species Homo sapiens (human)
Host Cell BEAS-2B
Host Cell Species Homo sapiens (Human)
Reporter GFP
Applications 1) investigation of gene function
2) screening and validation of antibodies
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Real-time qPCR analysis of gene mRNA overexpression level
2) GFP fluorescent detection under fluorescent microscopy
3) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene CLEC7A
Background This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]
Quick Inquiry

Q & A

Customer Reviews

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Customer Reviews
High-Quality Product

We found the Human CLEC7A Stable Cell Line - BEAS-2B to be highly dependable. It was particularly useful for studying host-pathogen interactions with excellent expression stability.

United States

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction