Cat.No. : CSC-RO0496 Host Cell: CHO-K1
| Cat. No. | CSC-RO0496 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Mouse ICOS. |
| Gene | ICOS |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Mouse ICOS Stable Cell Line - CHO-K1, due to its stable expression of the murine Immune costimulatory molecule (ICOS), has significant potential in studying T cell activation and regulation. ICOS is an important immunoregulatory molecule that promotes the proliferation and differentiation of T cells. This cell line can be used to deepen the understanding of the role of ICOS in T cell immune responses.
A: By using the Mouse ICOS Stable Cell Line - CHO-K1, the interaction between ICOS and its ligand can be studied through co-immunoprecipitation experiments. Additionally, cell binding experiments and flow cytometry can be used to assess the binding affinity and specificity of ICOS with its ligand.
A: For antibody screening, experiments should be designed to evaluate the binding capacity and functional impact of different antibodies on the ICOS protein in the Mouse ICOS Stable Cell Line - CHO-K1. ELISA, flow cytometry, or functional cell assays can be used to assess the efficiency of antibody binding and the modulation of the ICOS signaling pathway.
A: The Mouse ICOS Stable Cell Line - CHO-K1 has potential applications in the study of autoimmune diseases. ICOS plays a crucial role in regulating the function of T cells, especially helper T cells. This cell line can be used to study the regulatory mechanisms of ICOS in autoimmune diseases, providing targets for new therapeutic strategies.
A: When conducting gene editing experiments with the Mouse ICOS Stable Cell Line - CHO-K1, it is important to maintain the expression and function of the ICOS protein. The choice and design of gene editing tools, such as CRISPR/Cas9, should ensure specificity to the target gene and accurate validation of the editing effects.
A: The Mouse ICOS Stable Cell Line - CHO-K1 has promising prospects in tumor immunotherapy research. As a co-stimulatory molecule, ICOS plays an important role in regulating anti-tumor immune responses. This cell line can be used to study the role of ICOS in the tumor microenvironment and its potential as an immunotherapy target.
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