Mouse ICOS Stable Cell Line - CHO-K1

Name: Mouse ICOS Stable Cell Line - CHO-K1
SKU: CSC-RO0496
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0496

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0496
Description	This cell line is derived from CHO-K1 and is engineered to stably overexpress Mouse ICOS.
Target Gene	ICOS
Gene Species	Mus musculus (Mouse)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the potential application of the Mouse ICOS Stable Cell Line - CHO-K1 in studying T cell activation and regulation?

A: The Mouse ICOS Stable Cell Line - CHO-K1, due to its stable expression of the murine Immune costimulatory molecule (ICOS), has significant potential in studying T cell activation and regulation. ICOS is an important immunoregulatory molecule that promotes the proliferation and differentiation of T cells. This cell line can be used to deepen the understanding of the role of ICOS in T cell immune responses.

How can the Mouse ICOS Stable Cell Line - CHO-K1 be used to study the interaction between ICOS and its ligand?

A: By using the Mouse ICOS Stable Cell Line - CHO-K1, the interaction between ICOS and its ligand can be studied through co-immunoprecipitation experiments. Additionally, cell binding experiments and flow cytometry can be used to assess the binding affinity and specificity of ICOS with its ligand.

How should experiments be designed for antibody screening using the Mouse ICOS Stable Cell Line - CHO-K1?

A: For antibody screening, experiments should be designed to evaluate the binding capacity and functional impact of different antibodies on the ICOS protein in the Mouse ICOS Stable Cell Line - CHO-K1. ELISA, flow cytometry, or functional cell assays can be used to assess the efficiency of antibody binding and the modulation of the ICOS signaling pathway.

What is the potential application of the Mouse ICOS Stable Cell Line - CHO-K1 in studying autoimmune diseases?

A: The Mouse ICOS Stable Cell Line - CHO-K1 has potential applications in the study of autoimmune diseases. ICOS plays a crucial role in regulating the function of T cells, especially helper T cells. This cell line can be used to study the regulatory mechanisms of ICOS in autoimmune diseases, providing targets for new therapeutic strategies.

What key factors should be considered when conducting gene editing experiments with the Mouse ICOS Stable Cell Line - CHO-K1?

A: When conducting gene editing experiments with the Mouse ICOS Stable Cell Line - CHO-K1, it is important to maintain the expression and function of the ICOS protein. The choice and design of gene editing tools, such as CRISPR/Cas9, should ensure specificity to the target gene and accurate validation of the editing effects.

What are the prospects of the Mouse ICOS Stable Cell Line - CHO-K1 in tumor immunotherapy research?

A: The Mouse ICOS Stable Cell Line - CHO-K1 has promising prospects in tumor immunotherapy research. As a co-stimulatory molecule, ICOS plays an important role in regulating anti-tumor immune responses. This cell line can be used to study the role of ICOS in the tumor microenvironment and its potential as an immunotherapy target.

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