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Cat.No. : CSC-RO1017 Host Cell: HEK293T
| Cat. No. | CSC-RO1017 |
| Description | HEK293-CD24 cell line is engineered to stably overexpress monkey CD24. |
| Gene | CD24 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Ensure the use of appropriate cryopreservation media and thawing protocols, gradually adapt to culture conditions, and if necessary, add growth factors or serum to stimulate cell growth.
A: Try optimizing culture conditions, such as adjusting medium components, temperature, and CO₂ concentration, or consider using gene amplification techniques to enhance the expression of the CD24 gene.
A: Coat culture plates with poly-lysine or other cell adhesion promoters, or use special culture plates better suited for adherent cell growth.
A: Experiment with different transfection reagents like liposomes or electroporation, or optimize the quality of DNA and transfection conditions, such as DNA concentration and cell density.
A: Ensure the use of correct differentiation inducers and protocols, possibly adjusting the dosage or duration of treatment, or consider using co-culture systems to provide a more suitable microenvironment for differentiation.
A: Regularly monitor the genotype and phenotype of the cells, avoid over-passaging, and store early passage cells at low temperatures for future use.
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