Monkey CD24 Stable Cell Line-HEK293T

Name: Monkey CD24 Stable Cell Line-HEK293T
SKU: CSC-RO1017
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1017

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO1017
Description	HEK293-CD24 cell line is engineered to stably overexpress monkey CD24.
Target Gene	CD24
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How to deal with slow growth of Monkey CD24 Stable Cell Line-HEK293 after thawing from cryopreservation?

A: Ensure the use of appropriate cryopreservation media and thawing protocols, gradually adapt to culture conditions, and if necessary, add growth factors or serum to stimulate cell growth.

How to enhance the expression of CD24 protein in Monkey CD24 Stable Cell Line-HEK293?

A: Try optimizing culture conditions, such as adjusting medium components, temperature, and CO₂ concentration, or consider using gene amplification techniques to enhance the expression of the CD24 gene.

How to resolve the decreased cell adhesion in Monkey CD24 Stable Cell Line-HEK293 during high-throughput screening?

A: Coat culture plates with poly-lysine or other cell adhesion promoters, or use special culture plates better suited for adherent cell growth.

How to improve transfection efficiency in Monkey CD24 Stable Cell Line-HEK293?

A: Experiment with different transfection reagents like liposomes or electroporation, or optimize the quality of DNA and transfection conditions, such as DNA concentration and cell density.

How to improve the response in differentiation induction experiments with Monkey CD24 Stable Cell Line-HEK293?

A: Ensure the use of correct differentiation inducers and protocols, possibly adjusting the dosage or duration of treatment, or consider using co-culture systems to provide a more suitable microenvironment for differentiation.

How to maintain genetic stability in Monkey CD24 Stable Cell Line-HEK293 during long-term culture?

A: Regularly monitor the genotype and phenotype of the cells, avoid over-passaging, and store early passage cells at low temperatures for future use.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *