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Cat.No. : CSC-RO1019 Host Cell: HEK293T
| Cat. No. | CSC-RO1019 |
| Description | HEK293-CD226 cell line is engineered to stably overexpress monkey CD226. |
| Gene | CD226 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Monkey CD226 Stable Cell Line-HEK293 is created by introducing the CD226 gene into HEK293 cells, allowing them to stably express the CD226 protein. This is usually achieved through viral vector-mediated transfection or CRISPR/Cas9 gene editing technology.
A: This cell line can be used to study the role of CD226 in immune recognition, immune regulation, and immune diseases, helping to reveal the molecular mechanisms of immune responses.
A: Cell viability and stability can be assessed by detecting CD226 protein expression levels, cell growth curves, and cell cycle analysis.
A: This cell line can be used to screen and evaluate drugs targeting CD226 or its related pathways. By observing changes in CD226 expression or function after drug treatment, the potential efficacy and safety of drugs can be assessed.
A: During the experimental process, attention should be paid to aseptic techniques to avoid cell contamination. Also, it is important to regularly change the culture medium and maintain an appropriate cell density to ensure stable cell growth.
A: The cells can be stored in liquid nitrogen to maintain their stability. Before freezing, cells should be suspended in culture medium containing an appropriate concentration of cryoprotectant (such as DMSO) and gradually cooled in a programmable freezer. When thawing, quickly transfer the cells to a 37°C water bath and then add pre-warmed culture medium.
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