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Cat.No. : CSC-RO1061 Host Cell: CHO-S
| Cat. No. | CSC-RO1061 |
| Description | CHO-S-TREM1 cell line is engineered to stably overexpress human TREM1. |
| Gene | TREM1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-S |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The cell line was established by stably transfecting the human TREM1 (Triggering Receptor Expressed on Myeloid cells 1) gene into CHO-S cells.
A: This cell line can be used to study the role of TREM1 in inflammatory responses, including the release of inflammatory mediators and the activation of immune cells.
A: It can be used for screening and evaluating drugs targeting TREM1-mediated inflammatory responses, especially in treating autoimmune diseases and chronic inflammatory conditions.
A: With its ability to stably express human TREM1, this cell line can effectively simulate human disease processes related to TREM1, such as infectious diseases and certain types of cancers.
A: It needs to be maintained under specific culture conditions, including the use of a culture medium with selective antibiotics, to ensure the stable expression of the TREM1 gene.
A: It provides a valuable model for studying the role of TREM1 in the regulation of the immune system, especially in controlling the intensity and duration of immune responses.
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