Human SEMA4D Stable Cell Line - CHO-K1

Name: Human SEMA4D Stable Cell Line - CHO-K1
SKU: CSC-RO0878
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0878

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0878
Description	This cell line is engineered to stably overexpress human SEMA4D.
Target Gene	SEMA4D
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SEMA4D sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D [ Homo sapiens ]
Gene Symbol	SEMA4D
Synonyms	SEMA4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; C9orf164, chromosome 9 open reading frame 164 , SEMAJ; semaphorin-4D; CD100; coll 4; FLJ39737; M sema G; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; SEMAJ; coll-4; C9orf164; M-sema-G; FLJ33485; FLJ34282; FLJ46484; MGC169138; MGC169141;
Gene ID	10507
Uni Prot ID	Q92854
m RNA Refseq	BC054500
Chromosome Location	9q22-q31
Pathway	Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Sema4D in semaphorin signaling, organism-specific biosystem; Sema4D induced cell migration and growth-cone collapse, organism-specific biosystem; Sema4D mediated inhibition of cell attachment and migration, organism-specific biosystem;
MIM	601866

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