Cat.No. : CSC-RO0885 Host Cell: K562
| Cat. No. | CSC-RO0885 |
| Description | This cell line is engineered to stably overexpress membrane OKT3 (anti-CD3 monoclonal antibody) scFV sequence. |
| Gene | OKT3 scFV |
| Gene Species | Homo sapiens (Human) |
| Host Cell | K562 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human OKT3 scFV Stable Cell Line - K562 was established by stably transfecting the K562 cell line with the gene encoding the human OKT3 single-chain variable fragment (scFv). OKT3 is a monoclonal antibody targeting the CD3 complex on human T cells, widely used in immunoregulation research and therapy. This cell line aims to simulate the interaction between the OKT3 antibody and the human immune system, providing a model for studying T-cell-mediated immune response mechanisms and their application in immunotherapy.
A: The Human OKT3 scFV Stable Cell Line - K562, due to its expression of a single-chain antibody fragment against CD3, holds promise in the development of immunotherapies. Particularly in designing therapeutic strategies targeting specific T cell subpopulations, this cell line can serve as a research tool to help scientists evaluate and optimize the effects of immunomodulators, thereby offering new strategies for treating various immune-mediated diseases, including autoimmune diseases and cancer.
A: A distinctive feature of the Human OKT3 scFV Stable Cell Line - K562 is its stable expression of a single-chain variable fragment (scFv) with the same specificity as OKT3. This allows the cell line to simulate the specific binding of OKT3 antibodies to the CD3 complex on T cells in vitro. This capability is crucial for studying T cell activation, signal transduction, and the role of T cells in immune responses, providing a valuable tool for understanding antibody-mediated immunoregulatory mechanisms.
A: The establishment of the Human OKT3 scFV Stable Cell Line - K562, by expressing the OKT3 scFv, may affect its phenotype and function, especially regarding immune recognition and cell signaling pathways. The cell line may exhibit altered expression patterns of cell surface markers and may respond differently to external signals. These changes are significant for studying how specific immunomodulatory strategies can be used to activate or inhibit T cells.
A: The Human OKT3 scFV Stable Cell Line - K562 offers a unique platform for studying intercellular interactions, especially among immune cells. Through this cell line, the impact of OKT3 scFv on T cells and other immune cells like macrophages and B cells can be explored, paving new ways to understand cell signaling and immunoregulatory mechanisms.
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The Human OKT3 scFv Stable Cell Line - K562 has been genetically engineered to stably express single-chain variable fragment (scFv) antibodies, enabling efficient production of the CD3-targeting OKT3 antibody, which is valuable for immunotherapy and related research.
Utilizing the Human OKT3 scFv Stable Cell Line - K562 allows for direct antibody production and screening without the need for establishing complex recombinant antibody expression systems, significantly simplifying the antibody development process.
This cell line can stably and continuously produce specific antibodies, reducing the time spent on repeatedly constructing and validating expression systems, increasing the efficiency of research and development.
The expression of the OKT3 single-chain variable fragment (scFv) ensures high specificity and affinity, which is crucial for precise immune modulation and disease marker research.
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