Human OKT3 scFv Stable Cell Line - A549

Name: Human OKT3 scFv Stable Cell Line - A549
SKU: CSC-RO0516
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0516

Host Cell : A549 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0516
Description	This cell line is derived from A549 and is engineered to stably overexpress membrane OKT3 (anti-CD3 monoclonal antibody) scFV sequence.
Target Gene	OKT3 scFv
Gene Species	Homo sapiens (Human)
Host Cell	A549
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Customer Reviews

Customer Q&As

What are the unique applications of the Human OKT3 scFv Stable Cell Line - A549 in immunology research?

A: The Human OKT3 scFv Stable Cell Line - A549 expresses OKT3 single-chain variable fragments (scFv) to mimic the function of T cell receptors (TCRs). The uniqueness of this cell line lies in its ability to simulate T cell recognition and response to specific antigens, which is crucial for studying T cell-mediated immune response mechanisms. Additionally, as a lung cancer cell line, A549 can be used to investigate the role of T cells in the tumor microenvironment and develop new tumor immunotherapy strategies.

How does the Human OKT3 scFv Stable Cell Line - A549 assist in evaluating the immunogenicity of vaccines during vaccine development?

A: By introducing specific scFv into the Human OKT3 scFv Stable Cell Line - A549, researchers can simulate T cell responses induced by vaccines. This cell line can be used to assess whether vaccine candidates effectively activate T cells and whether this activation targets the correct antigens. This is crucial for ensuring the efficacy and safety of vaccines.

What is the role of the Human OKT3 scFv Stable Cell Line - A549 in studying T cell receptor signaling?

A: The Human OKT3 scFv Stable Cell Line - A549 provides a model system for studying the molecular mechanisms of T cell receptor (TCR) signaling. By expressing specific scFv in this cell line, researchers can investigate the activation of downstream signaling pathways following TCR activation, which is significant for understanding T cell function and regulation.

What is the role of the Human OKT3 scFv Stable Cell Line - A549 in studying T cell-mediated immune tolerance?

A: The Human OKT3 scFv Stable Cell Line - A549 can be used to study how T cells recognize self-tissues and maintain immune tolerance. By expressing specific scFv in this cell line, researchers can simulate T cell responses to self-antigens and investigate the mechanisms of immune tolerance, which is important for preventing the onset of autoimmune diseases.

What is the role of the Human OKT3 scFv Stable Cell Line - A549 in studying autoimmune diseases?

A: The Human OKT3 scFv Stable Cell Line - A549 can be used to simulate the abnormal T cell behavior in autoimmune diseases. By expressing specific scFv in this cell line, researchers can study how specific self-antigens are recognized by T cells and how this recognition leads to autoimmune responses. This is important for understanding the pathogenesis of autoimmune diseases and developing new treatment strategies.

How does the Human OKT3 scFv Stable Cell Line - A549 assist in assessing the potential impact of drugs on T cell function during drug screening?

A: By using the Human OKT3 scFv Stable Cell Line - A549, researchers can evaluate the impact of drugs on T cell receptor activation. This cell line can serve as a high-throughput screening platform for identifying compounds that may enhance or inhibit T cell activity. This is significant for developing new immunomodulatory drugs, especially those aimed at enhancing or suppressing T cell responses.

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