Cat.No. : CSC-RO0516 Host Cell: A549
| Cat. No. | CSC-RO0516 |
| Description | This cell line is derived from A549 and is engineered to stably overexpress membrane OKT3 (anti-CD3 monoclonal antibody) scFV sequence. |
| Gene | OKT3 scFv |
| Gene Species | Homo sapiens (Human) |
| Host Cell | A549 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human OKT3 scFv Stable Cell Line - A549 expresses OKT3 single-chain variable fragments (scFv) to mimic the function of T cell receptors (TCRs). The uniqueness of this cell line lies in its ability to simulate T cell recognition and response to specific antigens, which is crucial for studying T cell-mediated immune response mechanisms. Additionally, as a lung cancer cell line, A549 can be used to investigate the role of T cells in the tumor microenvironment and develop new tumor immunotherapy strategies.
A: By introducing specific scFv into the Human OKT3 scFv Stable Cell Line - A549, researchers can simulate T cell responses induced by vaccines. This cell line can be used to assess whether vaccine candidates effectively activate T cells and whether this activation targets the correct antigens. This is crucial for ensuring the efficacy and safety of vaccines.
A: The Human OKT3 scFv Stable Cell Line - A549 provides a model system for studying the molecular mechanisms of T cell receptor (TCR) signaling. By expressing specific scFv in this cell line, researchers can investigate the activation of downstream signaling pathways following TCR activation, which is significant for understanding T cell function and regulation.
A: The Human OKT3 scFv Stable Cell Line - A549 can be used to study how T cells recognize self-tissues and maintain immune tolerance. By expressing specific scFv in this cell line, researchers can simulate T cell responses to self-antigens and investigate the mechanisms of immune tolerance, which is important for preventing the onset of autoimmune diseases.
A: The Human OKT3 scFv Stable Cell Line - A549 can be used to simulate the abnormal T cell behavior in autoimmune diseases. By expressing specific scFv in this cell line, researchers can study how specific self-antigens are recognized by T cells and how this recognition leads to autoimmune responses. This is important for understanding the pathogenesis of autoimmune diseases and developing new treatment strategies.
A: By using the Human OKT3 scFv Stable Cell Line - A549, researchers can evaluate the impact of drugs on T cell receptor activation. This cell line can serve as a high-throughput screening platform for identifying compounds that may enhance or inhibit T cell activity. This is significant for developing new immunomodulatory drugs, especially those aimed at enhancing or suppressing T cell responses.
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