Human LICAM Stable Cell Line - HEK293T

Name: Human LICAM Stable Cell Line - HEK293T
SKU: CSC-RO0952
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0952

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0952
Description	This cell line is engineered to stably overexpress human LICAM.
Target Gene	LICAM
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

How is the Human LICAM Stable Cell Line - HEK293T constructed, and what is the scientific principle behind it?

A: The Human LICAM Stable Cell Line - HEK293T is created by introducing the LICAM gene into HEK293T cells, allowing them to stably express the LICAM protein. This is usually achieved through viral vector-mediated transfection or CRISPR/Cas9 gene editing technology.

What are the applications of Human LICAM Stable Cell Line - HEK293T in studying cell adhesion and signal transduction?

A: This cell line can be used to study the role of LICAM in cell adhesion, signal transduction, and cell migration processes, helping to reveal the molecular mechanisms of cellular behavior.

How can the cell viability and stability of Human LICAM Stable Cell Line - HEK293T be evaluated?

A: Cell viability and stability can be assessed by detecting LICAM protein expression levels, cell growth curves, and cell cycle analysis.

What is the use of Human LICAM Stable Cell Line - HEK293T in drug screening?

A: This cell line can be used to screen and evaluate drugs targeting LICAM or its related pathways. By observing changes in LICAM expression or function after drug treatment, the potential efficacy and safety of drugs can be assessed.

How can Human LICAM Stable Cell Line - HEK293T cells be stored to maintain their stability?

A: The cells can be stored in liquid nitrogen to maintain their stability. Before freezing, cells should be suspended in culture medium containing an appropriate concentration of cryoprotectant (such as DMSO) and gradually cooled in a programmable freezer. When thawing, quickly transfer the cells to a 37°C water bath and then add pre-warmed culture medium.

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