Human KLRD1/KLRC2 Stable Cell Line - CHO-K1

Name: Human KLRD1/KLRC2 Stable Cell Line - CHO-K1
SKU: CSC-RO0906
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0906

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0906
Description	This cell line is engineered to stably overexpress human KLRD1/KLRC2 (also named as CD94-NKG2A).
Target Gene	KLRD1/KLRC2
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the Human KLRD1/KLRC2 Stable Cell Line - CHO-K1, and what are its main characteristics?

A: The Human KLRD1/KLRC2 Stable Cell Line - CHO-K1 is a stable cell line constructed by transfecting CHO-K1 cell line with human KLRD1 (also known as CD94) and KLRC2 (also known as NKG2C) genes. KLRD1 and KLRC2 are components of natural killer cell receptors and play important roles in the immune system.

Why was the CHO-K1 cell line chosen to construct the Human KLRD1/KLRC2 Stable Cell Line?

A: The CHO-K1 cell line is a widely used mammalian cell line in biomedical research and production. It possesses stable chromosomal structure and is easy to culture, making it ideal for constructing stable transfected cell lines. Additionally, CHO-K1 cells lack endogenous expression of KLRD1 and KLRC2, making them an ideal choice for constructing the Human KLRD1/KLRC2 Stable Cell Line.

What is the role of KLRD1 and KLRC2 in the immune system?

A: KLRD1 and KLRC2 are receptors of natural killer cells (NK cells) and play crucial roles in the immune system. KLRD1, encoded by CD94, forms different receptor complexes with other molecules (such as NKG2A) to recognize HLA-E antigens on MHC-I molecules. KLRC2, encoded by NKG2C, recognizes HLA-E antigens associated with CMV (cytomegalovirus) infection. These receptors are essential for regulating NK cell activity and recognizing infected and tumor cells.

What are the potential applications of Human KLRD1/KLRC2 Stable Cell Line - CHO-K1?

A: The Human KLRD1/KLRC2 Stable Cell Line - CHO-K1 can be applied in various fields. It can be used to study the mechanisms of NK cell activity regulation, immune responses, and tumor immunology. Additionally, this cell line can be used for screening and evaluating potential immunotherapies, drug candidates, and vaccine efficacy, particularly for diseases or infections related to HLA-E.

Does the maintenance and cultivation of Human KLRD1/KLRC2 Stable Cell Line - CHO-K1 require special attention?

A: The maintenance and cultivation of Human KLRD1/KLRC2 Stable Cell Line - CHO-K1 typically require standard cell culture conditions similar to those used for CHO-K1 cell lines. This includes the choice of culture medium, temperature, CO2 concentration, and cell density management. Additionally, appropriate selective antibiotics should be added as needed to maintain stable expression of KLRD1 and KLRC2 genes.

Are there known diseases or pathological conditions associated with mutations or abnormal expression of KLRD1/KLRC2 genes?

A: Mutations or abnormal expression of KLRD1 and KLRC2 genes are associated with some immunological diseases and infections. For example, abnormal expression of KLRD1 and KLRC2 may be linked to autoimmune diseases, viral infections (such as CMV), and immune escape in tumors. Therefore, the Human KLRD1/KLRC2 Stable Cell Line - CHO-K1 can be used to study the mechanisms of these diseases and potential therapeutic approaches.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *