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Cat.No. : CSC-RO1052 Host Cell: HEK293T
| Cat. No. | CSC-RO1052 |
| Description | HEK293-IL4R cell line is engineered to stably overexpress human IL4R. |
| Gene | IL4R |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human IL4R Stable Cell Line-HEK293 originates from human embryonic kidney 293 (HEK293) cells that were transfected with a stable expression construct containing the human IL4R (Interleukin-4 receptor) gene. This gene encodes for the IL-4 receptor, a protein involved in immune responses and signaling. The cell line was established through a transfection and selection process to create stable cell populations expressing human IL4R.
A: IL-4 binding to its receptor IL4R can activate several downstream signaling pathways, including the JAK-STAT pathway, PI3K-AKT pathway, and MAPK pathway. These pathways play crucial roles in immune cell differentiation, proliferation, and cytokine production. Activation of these pathways in the Human IL4R Stable Cell Line-HEK293 can be studied to better understand the immunomodulatory effects of IL-4.
A: To maintain the Human IL4R Stable Cell Line-HEK293, it is typically cultured in a suitable growth medium containing high-glucose DMEM (Dulbecco's Modified Eagle Medium) supplemented with Fetal Bovine Serum (FBS) and antibiotics such as penicillin and streptomycin. The exact conditions may vary depending on the specific research goals, but maintaining a stable temperature (37°C) and CO2 concentration (5%) is standard.
A: IL-4R is known to interact with other receptors, such as IL-13 receptor subunits, leading to shared downstream signaling pathways. These interactions have implications in allergic and autoimmune diseases. In the context of immunotherapy and drug development, targeting IL-4R or its interactions may provide novel approaches for modulating immune responses.
A: Research with the Human IL4R Stable Cell Line-HEK293 can have translational applications in developing targeted therapies for conditions involving IL-4R dysregulation, such as allergic diseases and autoimmune disorders. By studying IL-4R signaling and the effects of potential drugs, researchers can contribute to the development of more effective treatments with fewer side effects.
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