Human IL4R Stable Cell Line-HEK293T

Name: Human IL4R Stable Cell Line-HEK293T
SKU: CSC-RO1052
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1052

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1052
Description	HEK293-IL4R cell line is engineered to stably overexpress human IL4R.
Target Gene	IL4R
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	IL4R interleukin 4 receptor [ Homo sapiens ]
Gene Symbol	IL4R
Synonyms	CD124; IL4RA; IL-4RA
Gene ID	3566
Uni Prot ID	P24394
m RNA Refseq	NM_000418.3
Protein Refseq	NP_000409.1
Chromosome Location	16p12.1-p11.2
Function	insulin receptor substrate binding; interleukin-4 receptor activity; protein binding; receptor signaling protein activity;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; IL-4 signaling Pathway, organism-specific biosystem; IL4-mediated signaling events, organism-specific biosystem; Inflammatory Response Pathway, organism-specific biosystem;
MIM	147781

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Q & A

Customer Reviews

Customer Q&As

What is the specific origin of the Human IL4R Stable Cell Line-HEK293, and how was it established?

A: The Human IL4R Stable Cell Line-HEK293 originates from human embryonic kidney 293 (HEK293) cells that were transfected with a stable expression construct containing the human IL4R (Interleukin-4 receptor) gene. This gene encodes for the IL-4 receptor, a protein involved in immune responses and signaling. The cell line was established through a transfection and selection process to create stable cell populations expressing human IL4R.

Can you provide insights into the downstream signaling pathways activated by IL-4 binding to the IL-4 receptor in the Human IL4R Stable Cell Line-HEK293?

A: IL-4 binding to its receptor IL4R can activate several downstream signaling pathways, including the JAK-STAT pathway, PI3K-AKT pathway, and MAPK pathway. These pathways play crucial roles in immune cell differentiation, proliferation, and cytokine production. Activation of these pathways in the Human IL4R Stable Cell Line-HEK293 can be studied to better understand the immunomodulatory effects of IL-4.

What specific experimental conditions and media are recommended for maintaining the Human IL4R Stable Cell Line-HEK293?

A: To maintain the Human IL4R Stable Cell Line-HEK293, it is typically cultured in a suitable growth medium containing high-glucose DMEM (Dulbecco's Modified Eagle Medium) supplemented with Fetal Bovine Serum (FBS) and antibiotics such as penicillin and streptomycin. The exact conditions may vary depending on the specific research goals, but maintaining a stable temperature (37°C) and CO2 concentration (5%) is standard.

Are there any known interactions between IL-4R and other cell surface receptors or molecules that may have implications in immunotherapy or drug development?

A: IL-4R is known to interact with other receptors, such as IL-13 receptor subunits, leading to shared downstream signaling pathways. These interactions have implications in allergic and autoimmune diseases. In the context of immunotherapy and drug development, targeting IL-4R or its interactions may provide novel approaches for modulating immune responses.

What are the potential translational applications of research involving the Human IL4R Stable Cell Line-HEK293 in the context of immunotherapy or allergy treatment?

A: Research with the Human IL4R Stable Cell Line-HEK293 can have translational applications in developing targeted therapies for conditions involving IL-4R dysregulation, such as allergic diseases and autoimmune disorders. By studying IL-4R signaling and the effects of potential drugs, researchers can contribute to the development of more effective treatments with fewer side effects.

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