Human IL2RA Stable Cell Line - CHO-S

Name: Human IL2RA Stable Cell Line - CHO-S
SKU: CSC-RO0864
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0864

Host Cell : CHO-S Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0864
Description	This cell line is engineered to stably overexpress human IL2RA (also named as CD25).
Target Gene	IL2RA
Gene Species	Homo sapiens (Human)
Host Cell	CHO-S
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	IL2RA interleukin 2 receptor, alpha [ Homo sapiens ]
Gene Symbol	IL2RA
Synonyms	CD25; IL2R; TCGFR; IDDM10
Gene ID	3559
Uni Prot ID	P01589
m RNA Refseq	NM_000417.2
Protein Refseq	NP_000408.1
Chromosome Location	10p15-p14
Function	interleukin-2 receptor activity;
Pathway	Arf6 trafficking events, organism-specific biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem;
MIM	147730

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What are the specific applications of the Human IL2RA Stable Cell Line - CHO-S in autoimmune research?

A: The Human IL2RA Stable Cell Line - CHO-S is primarily used for studying IL2RA-related autoimmune responses, such as in diabetes and immunodeficiency diseases research. Moreover, as IL2RA is associated with immune checkpoints, this cell line can also be used for drug screening and biological evaluation of immune checkpoint inhibitors.

How can the stability and reliability of the Human IL2RA Stable Cell Line - CHO-S be ensured during drug screening?

A: To ensure the stability and reliability of the cell line, regular mycoplasma testing is necessary to rule out contamination. Also, sterile testing is important to maintain sterility during cell passage. Additionally, it is recommended to culture the cells in a medium containing puromycin to maintain antibiotic resistance.

What are the optimal culture conditions for the Human IL2RA Stable Cell Line - CHO-S?

A: The optimal culture conditions for the Human IL2RA Stable Cell Line - CHO-S are at 37°C with 5% CO2, using EX-Cell CD CHO Fusion medium supplemented with 10μg/mL puromycin. This environment helps maintain optimal cell growth and functional activity.

What is the potential role of the Human IL2RA Stable Cell Line - CHO-S in Acute Myeloid Leukemia (AML) research?

A: IL2RA expression in AML is associated with chemotherapy resistance and poor prognosis, so the Human IL2RA Stable Cell Line - CHO-S could be used to study the role of IL2RA in AML cell proliferation, differentiation, and apoptosis. This cell line can be utilized to explore the biological role of IL2RA in promoting leukemia stem cell traits and leukemogenesis.

What are the specific steps for high-throughput drug screening using the Human IL2RA Stable Cell Line - CHO-S?

A: For high-throughput drug screening using the Human IL2RA Stable Cell Line - CHO-S, cells are first seeded in a 384-well plate and cultured overnight at 37°C with 5% CO2 to allow cell attachment and growth. Subsequently, cells are stimulated with control drugs for drug screening and functional evaluation.

What considerations are needed for the storage and transportation of the Human IL2RA Stable Cell Line - CHO-S?

A: The Human IL2RA Stable Cell Line - CHO-S is provided as frozen cells and should be stored in liquid nitrogen. During transportation, dry ice is used to maintain low temperatures. This ensures that the cells maintain the highest vitality and stability upon arrival in the laboratory.

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