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Human HLA-A2 Stable Cell Line - CFBE

Human HLA-A2 Stable Cell Line - CFBE

Cat.No. :  CSC-RO0649 Host Cell:  CFBE

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Cat. No. CSC-RO0649
Description This cell line is engineered to stably overexpress human HLA-A*02 (A*02) which is a human leukocyte antigen serotype within the HLA-A serotype group. HLA-A*02 is one particular class I major histocompatibility complex (MHC) allele group at the HLA-A locus.
Gene HLA-A2
Gene Species Homo sapiens (Human)
Host Cell CFBE
Host Cell Species Homo sapiens (Human)
Stability Validated for at least 10 passages
Application

1. Studying the interactions between immune cells and cancer cells

2. Studying the mechanisms of resistance to immune checkpoint blockade

3. High-throughput screening

4. Drug target validation

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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Human stable cell line HLA-A2. The major histocompatibility complex class I (MHCI) molecules, which include the human leukocyte antigen-A2 (HLA-A2) gene, are expressed by CFBE through engineering. Anchored in the cell membrane, HLA-A2 is an essential component of the immune system that helps cytotoxic T cells deliver peptides produced from intracellular proteins and thereby trigger antigen-specific immune responses against cells that are infected or altered. Eight exons make up the HLA-A gene, which is found on chromosome 6 and codes for the heavy chain of the MHCI molecule. Birdshot chorioretinopathy and severe cutaneous adverse responses are two conditions linked to the protein-coding gene HLA-A2. It contributes to immune surveillance and response by presenting a limited peptide repertoire made up of self-peptides, tumor peptide antigens, and viral epitopes. Adherent CFBE cells are commonly employed in research and are cultureable in vitro under conventional conditions. These cells have surface expression of HLA-A2, which makes it possible to analyze immunological responses and peptide presentation under controlled conditions.
1. HLA-A2 Gene Expression: To aid in the investigation of antigen presentation pathways, CFBE cells were genetically modified to express the HLA-A2 gene. 2. Immune Response Research: Examining immune responses specific to antigens against mutated or infected cells is essential to comprehending host defense mechanisms. 3. Gene Function Research: Examining HLA-A2's function in immune monitoring and response regulation to support the creation of immunotherapies. 4. Disease Association Study: Increasing our knowledge of autoimmune and hypersensitivity illnesses by examining the connections between HLA-A2, Birdshot chorioretinopathy, and severe cutaneous adverse responses. 5. Cytotoxic T Cell Interaction: Researching how HLA-A2 exposes cytotoxic T cells to a variety of self-peptides, tumor peptide antigens, and viral epitopes will help produce vaccines and enable tailored treatment.
Customer Q&As
How stable is this human HLA-A2 stable cell line? Is there long-term data support?

A: Our human HLA-A2 stable cell lines have been tested for long-term stability and maintain stable expression levels over multiple passages. We regularly validate cell lines and record cell line stability data. Customers can request this data from us to understand the stability performance of our products.

What is the expression level of this stable cell line when studying HLA-A2-mediated immune responses? Is relevant experimental data support provided?

A: Our human HLA-A2 stable cell lines express stable levels and are widely used in immune response research. We provide detailed experimental data support that customers can refer to to evaluate the performance of our products.

Is this human HLA-A2 stable cell line suitable for vaccine development or immunotherapy experiments? Is there relevant preclinical research data to support this?

A: Our human HLA-A2 stable cell lines have been validated in vaccine development and immunotherapy experiments. We provide relevant preclinical research data support to prove the application potential of our products in these fields. Customers can refer to these data to understand the performance and application scope of our products in preclinical studies.

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Customer Reviews
Reliable

The expression of HLA-A2 is very stable, which is crucial for studying antigen-specific immune responses mediated by cytotoxic T cells. The cells grew well in the culture medium with normal morphology and no abnormal changes, ensuring the reliability of experimental data. The cell products provided by the supplier are of excellent quality and the overall experience is very professional and reliable.

United States

05/21/2023

Recommended

The purchasing experience of Human HLA-A2 Stable Cell Line - CFBE was very smooth, from placing the order to receiving the goods, the entire process was efficient and transparent. The customer service staff are very professional and provide detailed technical support and answers. The cells grew well during the culture process without any contamination or abnormal problems, which ensured the smooth progress of the experiment. The gene expression of HLA-A2 is stable, ensuring the accuracy of experimental results. The platform provides detailed instructions for use, making the entire use process very smooth. Whether it is product quality or service level, this cell line is highly recommended.

Canada

07/16/2021

Ideal tool

While conducting research using the Human HLA-A2 Stable Cell Line - CFBE, I found that this cell line is very suitable for analyzing immune responses and antigen presentation. The cells grew rapidly in the culture medium and maintained good shape and vitality. Gene expression analysis shows that the expression of HLA-A2 is very stable, providing reliable data support for studying immune surveillance and response. CFBE cells have strong protein expression capabilities and easy operation, making them an ideal choice for complex immunological research. The supplier's delivery speed is fast and the packaging is professional, ensuring the viability of the cells.

Canada

10/10/2020

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