Human FGL1 Stable Cell Line - MC38

Name: Human FGL1 Stable Cell Line - MC38
SKU: CSC-RO0913
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0913

Host Cell : MC38 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0913
Description	This cell line is engineered to stably overexpress human FGL1.
Target Gene	FGL1
Gene Species	Homo sapiens (Human)
Host Cell	MC38
Host Cell Species	Mus musculus (Mouse)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	FGL1 fibrinogen-like 1 [ Homo sapiens ]
Gene Symbol	FGL1
Synonyms	HFREP1; HP-041; LFIRE1; LFIRE-1
Gene Description	fibrinogen-like 1
Gene ID	2267
Uni Prot ID	Q08830
m RNA Refseq	NM_004467.3
Protein Refseq	NP_004458.3
Chromosome Location	8p22
Function	receptor binding;
MIM	605776

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How was the Human FGL1 Stable Cell Line - MC38 generated?

A: It was created by genetically modifying the mouse MC38 cell line using a lentiviral vector carrying the human FGL1 sequence, resulting in a stable MC38 clone expressing exogenous human FGL1.

What are the main research applications of the Human FGL1 Stable Cell Line - MC38?

A: Primarily used for drug screening and biological assays, especially in studying the interaction of FGL1 with the immune checkpoint molecule LAG3 and its effect on antigen-specific T cell activation.

What are the important considerations when experimenting with the Human FGL1 Stable Cell Line - MC38?

A: It requires specific culture conditions, including RPMI1640 medium with puromycin and appropriate cell splitting ratios.

What is the uniqueness of the Human FGL1 Stable Cell Line - MC38 in immunological research?

A: Its importance lies in studying the role of FGL1 in tumor immune evasion and immune checkpoint therapies due to FGL1's involvement in immune suppression.

What is the application of the Human FGL1 Stable Cell Line - MC38 in immune checkpoint therapy research?

A: Useful for assessing drugs targeting the FGL1 and LAG3 interaction and their impact on antigen-specific T cell activation.

What are the specific requirements for culturing and storing the Human FGL1 Stable Cell Line - MC38?

A: Cells should be stored in liquid nitrogen and properly resuscitated and cultured prior to use, maintained under recommended conditions.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *