Human ERBB2-CD274 Stable Cell Line - MC38

Name: Human ERBB2-CD274 Stable Cell Line - MC38
SKU: CSC-RO0870
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0870

Host Cell : MC38 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0870
Description	This cell line is engineered to stably overexpress human ERBB2-CD274 (also named as ERBB2-PDL1).
Target Gene	ERBB2-CD274
Gene Species	Homo sapiens (Human)
Host Cell	MC38
Host Cell Species	Mus musculus (Mouse)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

In immunotherapy research, has Human ERBB2-CD274 Stable Cell Line - MC38 been cited in literature or research reports?

A: This cell line has been cited in multiple research papers and research reports in the field of immunotherapy, where it has been used to investigate the effectiveness and mechanisms of immunotherapy strategies.

How can Human ERBB2-CD274 Stable Cell Line - MC38 simulate immune evasion phenomena in the tumor microenvironment?

A: By introducing ERBB2 and CD274 genes, along with appropriate cytokines, this cell line can simulate immune evasion phenomena in the tumor microenvironment, enabling the study of related mechanisms.

In tumor models, how is Human ERBB2-CD274 Stable Cell Line - MC38 used to study immunotherapy strategies?

A: This cell line can be used to establish tumor models and evaluate the effectiveness of immunotherapy strategies, including T cell infiltration detection and tumor growth inhibition.

What are the cell culture conditions for Human ERBB2-CD274 Stable Cell Line - MC38?

A: Human ERBB2-CD274 Stable Cell Line - MC38 typically needs to be maintained under specific culture medium and conditions, which may be optimized based on experimental requirements.

What are the potential applications of Human ERBB2-CD274 Stable Cell Line - MC38 in cancer immunotherapy research?

A: This cell line can be used to study the roles of ERBB2 and CD274 in cancer immunotherapy and evaluate the effectiveness of treatment strategies targeting these molecules.

What is the source of Human ERBB2-CD274 Stable Cell Line - MC38, and how is it constructed?

A: Human ERBB2-CD274 Stable Cell Line - MC38 is typically constructed by stably introducing the human ERBB2 and CD274 genes into MC38 cells. This can be achieved through techniques such as transfection or viral transduction.

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