Human CLEC9A Stable Cell Line - CHO-K1

Name: Human CLEC9A Stable Cell Line - CHO-K1
SKU: CSC-RO0950
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0950

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Cat. No.	CSC-RO0950
Description	This cell line is engineered to stably overexpress human CLEC9A.
Target Gene	CLEC9A
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CLEC9A C-type lectin domain family 9, member A [ Homo sapiens ]
Gene Symbol	CLEC9A
Synonyms	DNGR1; UNQ9341
GeneID	283420
Uni ProtID	Q6UXN8
mRNA Refseq	NM_207345.2
Protein Refseq	NP_997228.1
Chromosome Location	12p13.2
Function	carbohydrate binding;
MIM	612252

CSC-RO0950-Human CLEC9A Stable Cell Line - CHO-K1-Datasheet

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How was the Human CLEC9A Stable Cell Line - CHO-K1 developed?

A: The Human CLEC9A Stable Cell Line - CHO-K1 was developed through genetic engineering techniques by introducing the human CLEC9A gene into CHO-K1 cells, enabling stable expression of the CLEC9A protein.

What are the main research fields for the Human CLEC9A Stable Cell Line - CHO-K1?

A: It is used for studying the role of CLEC9A in immune regulation, antigen presentation, and inflammatory responses.

What should be considered when culturing the Human CLEC9A Stable Cell Line - CHO-K1?

A: Culturing should be conducted under specific conditions, including appropriate temperature, pH value, and culture medium.

What are the applications of the Human CLEC9A Stable Cell Line - CHO-K1 in drug development?

A: It can be used for screening and validating drugs targeting CLEC9A, such as antibody drugs.

What role does the Human CLEC9A Stable Cell Line - CHO-K1 play in cell signaling research?

A: It is used to study the intracellular signaling pathways activated by CLEC9A.

What is the significance of the Human CLEC9A Stable Cell Line - CHO-K1 in immune cell function research?

A: Due to CLEC9A's close association with the activation and regulation of immune cells, this cell line is particularly important for studying the functions of immune cells.

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