VspI

Name: VspI
SKU: EROT0489

For research use only. Not intended for any clinical use.

Cat.No.

EROT0489

Description

Vibrio species.

Concentration

8–12u/μl

Reaction Conditions

Buffer D. 37 °C.

Recognition Site

AT↓TAAT

Size/Form

500 units

Storage

20mM Tris-HCl (pH 7.5), 50mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.

Publications

Q & A

Customer Reviews

Customer Q&As

What is the methylation sensitivity of VspI?

A: VspI is not sensitive to Dam methylation, Dcm methylation, or CpG methylation.

What is the optimal reaction temperature for VspI?

A: Optimal reaction temperature is 37°C.

What is the optimal DNA concentration in restriction enzyme digestion?

A: The optimal range of DNA concentration is 0.02-0.1 μg/μL in the restriction digestion mixture. We would recommend the volume of the DNA sample not to exceed 30% of the total reaction volume to avoid potential reaction inhibition. To resolve inhibition from impurities of the DNA solution, we recommend increasing the overall volume of the reaction while keeping the volume of the DNA solution the same.

What are possible reasons for incomplete/failed restriction digestion?

A: The main reason for DNA cleavage reaction failure is the presence of contaminating inhibitors in the template DNA (for example: phenol, chloroform, detergents, ethanol, excess salts, EDTA, etc.).

Why do you recommend only 2 µL of 10X Reaction Buffer when digesting unpurified PCR product in a 30 µL reaction?

A: We recommend only 2 μl 10X Buffer in digestion of unpurified PCR products in 30 ul since salts and ions from the PCR reaction would be carried over to the digestion reaction.

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