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PaeI

For research use only. Not intended for any clinical use.
Cat.No.
EROT0427
Reaction Buffer
10mM Tris-HCl (pH 7.5 at 37 °C), 10mM MgCl2 and 0.1mg/mL BSA
Reaction Conditions
Incubate at 37 °C
Recognition Site
GCATG↓C
Size/Form
500 Units;1000 Units
Storage
10mM potassium-phosphate (pH 7.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.5mg/mL BSA and 50% (v/v) glycerol

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Q & A

Customer Reviews

Customer Q&As
What is the recognition site for PaeI?

A: PaeI restriction endonuclease recognizes the GCATGC^C site and exhibits optimal cutting efficiency at 37°C for 5-15 minutes in universal FastDigest buffer.

Under what conditions does PaeI exhibit optimal cutting efficiency?

A: No, shorter incubation times and the composition of the optimized universal FastDigest buffer eliminate star activity effects.

What are the advantages and characteristics of PaeI?

A: It is compatible with 100% of downstream applications and can complete digestion in 5-15 minutes, allowing direct loading onto gels.

How can PaeI be applied?

A: PaeI enzyme can be used in applications such as molecular cloning, restriction mapping, gene typing, Southern blotting, and SNP analysis, among others.

What are the components of the universal buffer for PaeI?

A: FastDigest and FastDigest Green buffers are proprietary digestion buffers that support 100% activity for all FastDigest restriction enzymes.

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