Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0427
| Cat. No. | EROT0427 |
| Recognition Site | GCATG↓C |
| Size | 500 Units;1000 Units |
| Reaction Buffer | 10mM Tris-HCl (pH 7.5 at 37 °C), 10mM MgCl2 and 0.1mg/mL BSA |
| Reaction Conditions | Incubate at 37 °C |
| Storage | 10mM potassium-phosphate (pH 7.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.5mg/mL BSA and 50% (v/v) glycerol |
A: PaeI restriction endonuclease recognizes the GCATGC^C site and exhibits optimal cutting efficiency at 37°C for 5-15 minutes in universal FastDigest buffer.
A: No, shorter incubation times and the composition of the optimized universal FastDigest buffer eliminate star activity effects.
A: It is compatible with 100% of downstream applications and can complete digestion in 5-15 minutes, allowing direct loading onto gels.
A: PaeI enzyme can be used in applications such as molecular cloning, restriction mapping, gene typing, Southern blotting, and SNP analysis, among others.
A: FastDigest and FastDigest Green buffers are proprietary digestion buffers that support 100% activity for all FastDigest restriction enzymes.
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