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Cat.No. : CSC-RO0196 Host Cell: CHO-K1
| Cat. No. | CSC-RO0196 |
| Description | This cell line is engineered to stably overexpress the monkey PVR in CHO-K1 cells. |
| Introduction | This cell line is constructed by transfection of monkey poliovirus receptor (PVR) into CHO-K1, followed by stable cell selection. The expression of PVR has been analyzed through flow cytometry. |
| Gene | PVR |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Modify the surface treatment of culture plates, such as coating with collagen or other cell adhesion promoters, or optimize culture conditions to enhance cell adhesion.
A: Use antibodies with high specificity and affinity, optimize the antibody dilution ratio and cell incubation conditions, and ensure cells are well-maintained during processing and analysis.
A: Optimize the design of sgRNA and the expression level of Cas9, increase the accessibility of the target sequence, and ensure the use of efficient transfection methods.
A: Optimize cell lysis conditions, such as using lysis buffer more suitable for PVR protein, ensure thorough lysis and appropriate centrifugation.
A: Check and optimize the composition of the culture medium, such as the concentration of serum and growth factors, and maintain a suitable culture environment, such as temperature and CO₂ concentration.
A: Optimize protein loading and transfer conditions, ensure the use of high-quality antibodies and appropriate detection methods, such as enhanced chemiluminescence.
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