Monkey PVR Stable Cell Line - CHO-K1

Name: Monkey PVR Stable Cell Line - CHO-K1
SKU: CSC-RO0196
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0196

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0196
Description	This cell line is engineered to stably overexpress the monkey PVR in CHO-K1 cells.
Introduction	This cell line is constructed by transfection of monkey poliovirus receptor (PVR) into CHO-K1, followed by stable cell selection. The expression of PVR has been analyzed through flow cytometry.
Target Gene	PVR
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

How to deal with the reduced cell adhesion in Monkey PVR Stable Cell Line - CHO-K1 during cell adhesion experiments?

A: Modify the surface treatment of culture plates, such as coating with collagen or other cell adhesion promoters, or optimize culture conditions to enhance cell adhesion.

How to improve the detection efficiency of surface PVR protein in flow cytometry analysis of Monkey PVR Stable Cell Line - CHO-K1?

A: Use antibodies with high specificity and affinity, optimize the antibody dilution ratio and cell incubation conditions, and ensure cells are well-maintained during processing and analysis.

How to enhance CRISPR/Cas9 gene editing efficiency in Monkey PVR Stable Cell Line - CHO-K1?

A: Optimize the design of sgRNA and the expression level of Cas9, increase the accessibility of the target sequence, and ensure the use of efficient transfection methods.

How to improve protein extraction efficiency when preparing protein samples from Monkey PVR Stable Cell Line - CHO-K1?

A: Optimize cell lysis conditions, such as using lysis buffer more suitable for PVR protein, ensure thorough lysis and appropriate centrifugation.

What solutions are there for slow cell growth in proliferation experiments with Monkey PVR Stable Cell Line - CHO-K1?

A: Check and optimize the composition of the culture medium, such as the concentration of serum and growth factors, and maintain a suitable culture environment, such as temperature and CO₂ concentration.

How to improve the stability of PVR protein signal in Western Blot experiments with Monkey PVR Stable Cell Line - CHO-K1?

A: Optimize protein loading and transfer conditions, ensure the use of high-quality antibodies and appropriate detection methods, such as enhanced chemiluminescence.

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