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Cat.No. : CSC-RT0033
Host Cell: MCF10A Target Gene: TP53
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0033 |
| Description | MCF10A-TP53 (-/-) is a cell line with a homozygous knockout of human TP53 |
| Target Gene | TP53 |
| Host Cell | MCF10A |
| Host Cell Species | Homo sapiens (Human) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
This reagent knockdown is very stable, as evidenced by the results of my western blot, which are authentic and trustworthy.
United States
03/11/2023
This knockout reagent is relatively easy to use, there are no other tedious steps and the results are very clear
United States
03/18/2023
I can responsibly say that this reagent works better than the foreign brands I have used before, where I could not run the correct trend and the model failed several times
United States
03/15/2023
This reagent has been a beacon of light in my experimental path, and I don't pull the trigger.
United States
01/16/2023
The results of the knockout were excellent and I can say that I did not envisage the results myself.
United States
02/19/2023
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