Human TP53 Knockout Cell Line-DLD-1

Name: Human TP53 Knockout Cell Line-DLD-1
SKU: CSC-RT0009
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0009

Target Gene : TP53 Host Cell : DLD-1

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0009
Description	DLD1-TP53 (-/-) is a cell line with a homozygous knockout of human TP53
Target Gene	TP53
Host Cell	DLD-1
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TP53 tumor protein p53 [ Homo sapiens ]
Gene Symbol	TP53
Synonyms	P53; BCC7; LFS1; TRP53
Gene Description	tumor protein p53 (Li-Fraumeni syndrome)
Gene ID	7157
Uni Prot ID	P04637
m RNA Refseq	NM_001126115.1
Protein Refseq	NP_001119587.1
Chromosome Location	17p13.1
Function	ATP binding; DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II transcription factor binding; RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; chaperone binding; chromatin binding; copper ion binding; damaged DNA binding; enzyme binding; histone acetyltransferase binding; histone deacetylase regulator activity; identical protein binding; p53 binding; protease binding; protein N-terminus binding; protein binding; protein heterodimerization activity; protein kinase binding; protein phosphatase 2A binding; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding; zinc ion binding;
Pathway	AMPK signaling, organism-specific biosystem; Activation of BH3-only proteins, organism-specific biosystem; Activation of NOXA and translocation to mitochondria, organism-specific biosystem; Activation of PUMA and translocation to mitochondria, organism-specific biosystem; Alzheimers Disease, organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem;
MIM	191170

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Speed up the process

This is the reagent company recommended by my teacher, and the reagents I used before have been unsatisfactory many times, and the results this time are very good.

United States

2021-12-02

Good effect

I have used this brand of antibody in previous experiments and the effect is very good, but I did not expect that the effect of this knockout reagent is so amazing.

United States

2022-02-06

Knockout succeed

I used a variety of methods to detect that this gene knockout was successful, and finally, my time and energy were not in vain.

United States

2022-09-10

Good result

I am very confident in my results, because using this product, I also have the questions of the reviewers.

United States

2023-01-18

Good result

My classmates and I very much agree with the results of this experiment, because we both ran it at the same time, and the results were very similar.

United States

2023-02-21

Good repeatability

After doing experiments with this reagent, I became more and more confident in myself, the results were very clear and the reproducibility was particularly good.

United States

2023-02-28

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Human TP53 Knockout Cell Line-DLD-1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Speed up the process

Good effect

Knockout succeed

Good result

Good result

Good repeatability

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