Cat.No. : CSC-RO0061 Host Cell: HEK293T
| Cat. No. | CSC-RO0061 |
| Description | This cell line is a stably transfected cell line which expresses human Toll-like receptor 10 (TLR10) with an N-terminal HA tag. |
| Background | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is most highly expressed in lymphoid tissues such as spleen, lymph node, thymus, and tonsil. |
| Gene | TLR10 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human TLR10 Stable Cell Line-HEK293 is specifically designed to express TLR10, making it a unique tool for studying the role of this receptor in immune responses. This cell line can be used to investigate the activation of immune cells, the production of cytokines, and the regulation of inflammatory responses, which are critical aspects of immunology research.
A: To maintain the stability and functionality of the Human TLR10 Stable Cell Line-HEK293, it is essential to follow optimized culture conditions, including appropriate growth media, temperature, and CO2 levels. Regular monitoring for mycoplasma contamination and periodic testing for TLR10 expression are necessary to ensure the cell line retains its intended characteristics.
A: The Human TLR10 Stable Cell Line-HEK293 can be utilized in high-throughput screening assays to identify compounds that modulate TLR10 activity, which can lead to the development of new therapeutics for diseases where TLR10 plays a significant role, such as autoimmune disorders and infectious diseases.
A: The use of the Human TLR10 Stable Cell Line-HEK293 in research must adhere to ethical guidelines that protect human dignity and welfare. This includes obtaining informed consent for the use of human-derived materials, ensuring that the cell line is used for research purposes only, and following guidelines set by institutional review boards (IRBs) and relevant regulatory bodies.
A: The Human TLR10 Stable Cell Line-HEK293 enables researchers to investigate the mechanisms by which TLR10 contributes to immune tolerance and its dysregulation in autoimmune diseases. This can lead to the identification of new therapeutic targets and strategies for the treatment of autoimmune disorders.
A: The Human TLR10 Stable Cell Line-HEK293 is genetically engineered to express the Toll-like receptor 10 (TLR10), which is a member of the Toll-like receptor family involved in the innate immune response. This cell line allows researchers to investigate the activation and downstream signaling pathways of TLR10 in response to various stimuli, contributing to a better understanding of immune defense mechanisms.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
