Cat.No. : CSC-RO0051 Host Cell: CHO-K1
| Cat. No. | CSC-RO0051 |
| Description | This cell line is a stably transfected cell line which expresses Human SIRPA without any tag. |
| Gene | SIRPA |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | SIRPA signal-regulatory protein alpha [ Homo sapiens ] |
| Gene Symbol | SIRPA |
| Synonyms | BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1 |
| Gene Description | signal-regulatory protein alpha |
| Gene ID | 140885 |
| UniProt ID | P78324 |
| mRNA Refseq | NM_001040022.1 |
| Protein Refseq | NP_001035111.1 |
| Chromosome Location | 20p13 |
| Function | SH3 domain binding; |
| Pathway | Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Hemostasis, organism-specific biosystem; IL-1 Signaling Pathway, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem; Prolactin Signaling Pathway, organism-specific biosystem; |
| MIM | 602461 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The interaction between immune cells and tumor cells is thought to be the mechanism by which tumor cells evade immune surveillance. To study this interaction, the researchers created a Human SIRPA Stable Cell Line-CHO. SIRPα, an Ig superfamily protein, inhibits phagocytosis in macrophages by binding to CD47 on target cells. In their study, they found that high levels of SIRPα expression were found in both melanoma and renal cell carcinoma in humans. By blocking SIRPα-CD47 interaction, an anti-SIRPα antibody suppressed tumor formation in immunocompetent mice through enhanced phagocytosis and signaling blockade. This dual mechanism suggests anti-SIRPα antibodies could enhance immune responses against cancers, potentially working in harmony with other therapeutic antibodies like CD20 and PD-1 inhibitors.
Figure 1. In mouse models of renal cell carcinoma and melanoma, the researchers show that antibodies against SIRPα inhibit tumor growth. This is supported by data on tumor volume and survival and is verified by immunoblot and flow cytometric analyses of SIRPα expression. (Yanagita T, et al., 2017)
A: Our human SIRPA stable cell lines undergo rigorous purification and validation procedures to ensure their purity and stability. We use a variety of techniques to identify cell lines, including PCR, immunofluorescence staining, and Western blot. We also regularly perform cytological testing of cell lines to ensure they are free of hybridization or mutation.
A: Our human SIRPA stable cell lines express stable levels and are widely used in cell signaling research. We provide detailed experimental protocols and technical support to help customers use our products in their research. In addition, we also provide relevant experimental data support, which customers can refer to to evaluate the performance of our products.
A: Our human SIRPA stable cell lines have been validated in in vitro and in vivo experiments. Although it is mainly used for in vitro cell research, it can also be used for experiments with certain primary cells. We provide detailed application guidance, including recommended experimental conditions and technical tips, to help customers get the most out of our products in their research.
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This cell line provides an ideal platform for SIRPA research, especially in the study of signal transduction pathways and receptor tyrosine kinases. The supplier's service was also very good, and timely communication and technical support made the experiment go more smoothly. Key parameters such as cell proliferation rate and protein expression are ideal.
I am really satisfied with the Human SIRPA Stable Cell Line-CHO from this platform. From placing the order to receiving the goods, the entire process was very smooth, and the customer service staff's professional knowledge and patient answers were impressive.
I used Human SIRPA Stable Cell Line-CHO to conduct research on SIRPA function, and the effect was very good. The cells grew rapidly in the culture medium and maintained good shape and vitality. Gene expression analysis showed that SIRPA expression was stable, which provided reliable data support for my signal transduction research. The high protein expression capabilities and ease of operation of CHO cells make them ideal for experiments.
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