Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-0832 Host Cell : HEK293
| Cat. No. | CLOE-0832 |
| Description | This cell lysate over-expresses his tagged human SIRPA (NP_542970.1) (Met 1-Arg 370). Purification of target protein (s) from this cell lysate has been verified. |
| Introduction | This cell lysate over-expresses his tagged human SIRPA (NP_542970.1) (Met 1-Arg 370). Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | SIRPA |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | SIRPA signal-regulatory protein alpha [ Homo sapiens ] |
| Gene Symbol | SIRPA |
| Synonyms | BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1 |
| Gene Description | signal-regulatory protein alpha |
| Gene ID | 140885 |
| Uni Prot ID | P78324 |
| m RNA Refseq | NM_001040022.1 |
| Protein Refseq | NP_001035111.1 |
| Chromosome Location | 20p13 |
| Function | SH3 domain binding; |
| Pathway | Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Hemostasis, organism-specific biosystem; IL-1 Signaling Pathway, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem; Prolactin Signaling Pathway, organism-specific biosystem; |
| MIM | 602461 |
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