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Human LAP3 Knockout Cell Line-A549

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT2134

Target Gene :   LAP3 Host Cell :   A549

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT2134
Description This cell is a stable cell line with a homozygous knockout of human LAP3 using CRISPR/Cas9.
Target Gene LAP3
Host Cell A549
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Storage Liquid nirtogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name LAP3 leucine aminopeptidase 3 [ Homo sapiens ]
Gene Symbol LAP3
Synonyms LAP; PEPS; LAPEP
Gene Description leucine aminopeptidase 3
Gene ID 51056
Uni Prot ID P28838
m RNA Refseq NM_015907.2
Protein Refseq NP_056991.2
Chromosome Location 4p15.32
Function aminopeptidase activity; magnesium ion binding; manganese ion binding; metalloexopeptidase activity; zinc ion binding;
Pathway Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, conserved biosystem;
MIM 170250
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
High-quality

Using this KO cell line is the fastest and most cost-effective option to accelerate my project. Very nice!

United States

Easy to use

I was able to quickly select the right cell line for my needs. Their service quality is high and their products are effective.

United States

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