Cat.No. : CSC-RO0105 Host Cell: HEK293T
| Cat. No. | CSC-RO0105 |
| Description | HEK293T-ICOS cell line is a stably transfected cell line which expresses human inducible T-cell costimulator (ICOS). |
| Gene | ICOS |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human ICOS Stable Cell Line-HEK293T is derived from the HEK293T cell line, which is a human embryonic kidney cell line. It is a widely used cell line in biomedical research.
A: The Human ICOS Stable Cell Line-HEK293T is commonly used in research to study the biology of ICOS (Inducible T-cell COStimulator) and its interactions with other molecules. It can be used to investigate ICOS signaling pathways, screen for potential drug candidates, and assess the impact of ICOS on immune responses.
A: When working with the Human ICOS Stable Cell Line-HEK293T, researchers should adhere to ethical guidelines for cell culture and research. This includes obtaining proper informed consent for the use of human-derived cells and complying with institutional and regulatory protocols, such as those related to biosafety and human research ethics.
A: The recommended storage for the Human ICOS Stable Cell Line-HEK293T typically involves cryopreservation in liquid nitrogen. When reviving the cells, they are usually thawed quickly and cultured in suitable growth medium with the appropriate supplements.
A: Researchers interested in working with the Human ICOS Stable Cell Line-HEK293T can consult scientific journals, cell culture protocols, and resources provided by cell culture suppliers and research institutions for guidance on best practices and experimental techniques involving this cell line. Additionally, online databases and forums may offer valuable insights and advice from experienced researchers in the field.
A: The specific genotype and phenotype of the Human ICOS Stable Cell Line-HEK293T would depend on the genetic modifications introduced to create the stable cell line. Typically, it would express the ICOS gene stably and may have additional genetic modifications for selection markers or reporter genes.
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