Human ICOS Stable Cell Line-HEK293T

Name: Human ICOS Stable Cell Line-HEK293T
SKU: CSC-RO0105
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0105

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0105
Description	HEK293T-ICOS cell line is a stably transfected cell line which expresses human inducible T-cell costimulator (ICOS).
Target Gene	ICOS
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens inducible T-cell co-stimulator DNA.
Gene Symbol	ICOS
Synonyms	AILIM, CD278, MGC39850
m RNA Refseq	NM_012092.2

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Q & A

Customer Reviews

Customer Q&As

What is the origin of the Human ICOS Stable Cell Line-HEK293T?

A: The Human ICOS Stable Cell Line-HEK293T is derived from the HEK293T cell line, which is a human embryonic kidney cell line. It is a widely used cell line in biomedical research.

What are the primary applications of the Human ICOS Stable Cell Line-HEK293T in research?

A: The Human ICOS Stable Cell Line-HEK293T is commonly used in research to study the biology of ICOS (Inducible T-cell COStimulator) and its interactions with other molecules. It can be used to investigate ICOS signaling pathways, screen for potential drug candidates, and assess the impact of ICOS on immune responses.

What regulatory and ethical considerations should be taken into account when working with the Human ICOS Stable Cell Line-HEK293T?

A: When working with the Human ICOS Stable Cell Line-HEK293T, researchers should adhere to ethical guidelines for cell culture and research. This includes obtaining proper informed consent for the use of human-derived cells and complying with institutional and regulatory protocols, such as those related to biosafety and human research ethics.

What are the recommended storage and revival procedures for the Human ICOS Stable Cell Line-HEK293T?

A: The recommended storage for the Human ICOS Stable Cell Line-HEK293T typically involves cryopreservation in liquid nitrogen. When reviving the cells, they are usually thawed quickly and cultured in suitable growth medium with the appropriate supplements.

Can you recommend any specific resources or references for researchers interested in working with the Human ICOS Stable Cell Line-HEK293T?

A: Researchers interested in working with the Human ICOS Stable Cell Line-HEK293T can consult scientific journals, cell culture protocols, and resources provided by cell culture suppliers and research institutions for guidance on best practices and experimental techniques involving this cell line. Additionally, online databases and forums may offer valuable insights and advice from experienced researchers in the field.

What is the specific genotype and phenotype of the Human ICOS Stable Cell Line-HEK293T?

A: The specific genotype and phenotype of the Human ICOS Stable Cell Line-HEK293T would depend on the genetic modifications introduced to create the stable cell line. Typically, it would express the ICOS gene stably and may have additional genetic modifications for selection markers or reporter genes.

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