Human GABRA4 Stable Cell Line-HEK293

Name: Human GABRA4 Stable Cell Line-HEK293
SKU: CSC-RI0007
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0007

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0007
Description	This cell line is engineered to overexpress human GABRA4
Target Gene	GABRA4
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuGABRA4
Alias	GABRA4, alpha 4
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 4 DNA.
Gene Symbol	GABRA4
Synonyms	GABRA4
mRNA Refseq	BC035055

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Background

Q & A

Customer Reviews

Gamma-aminobutyric acid (GABA)-gated ion channels are widely distributed in the mammalian brain and are major mediators of inhibitory synaptic transmission. A typical GABA ion channel has a pentameric structure consisting of 5 protein subunits, often alpha, beta and gamma or delta, combining to form a central ion conducting pore across the cell membrane. In humans there are six genes that encode alpha subunits, three that encode beta, three that encode gamma, and an additional seven genes that encode other subunits whose function is less-well understood than the alpha, beta and gamma subunits. GABA ion channels open and close in response to secretion of GABA from presynaptic terminals. GABAA alpha4 channels comprised of alpha4, beta3, and gamma2 subunits are extrasynaptic, estimated to represent<5% of="" all="" gabaa="" receptors="" located="" in="" the="" brain,="" and="" insensitive="" to="" benzodiazepines.="" research="" interest="" has="" focused="" on="" alpha4-containing="" receptors="" because="" they="" are="" potentiated="" by="" concentrations="" of="" ethanol="" experienced="" by="" human="" drinkers="" (1-3="" mm);="" one="" study="" showed="" this="" effect="" requires="" the="" delta="" subunit="" is="" part="" of="" the="">

Customer Q&As

What type of cells is the Human GABRA4 Stable Cell Line-HEK293 based on?

A: This cell line is based on HEK293 cells, with the human GABRA4 (gamma-aminobutyric acid A receptor alpha4 subunit) gene stably transfected into these cells.

What are the primary research applications of the Human GABRA4 Stable Cell Line-HEK293?

A: It is mainly used for studying the role of GABRA4 in neural system functions, especially in neural transmission and emotional regulation.

What is the application of the Human GABRA4 Stable Cell Line-HEK293 in drug discovery?

A: It can be used for screening drugs that affect GABRA4 activity, particularly in the treatment of neurological diseases and mood disorders.

What experimental procedures should be noted when using the Human GABRA4 Stable Cell Line-HEK293?

A: The cells should be handled under sterile conditions, with regular monitoring of cell growth and GABRA4 protein expression levels.

What are the culture conditions for the Human GABRA4 Stable Cell Line-HEK293?

A: They should be cultured at 37°C in a 5% CO2 environment, using a medium suitable for the growth of HEK293 cells.

What is the application of the Human GABRA4 Stable Cell Line-HEK293 in neuropharmacological research?

A: It can be used to study the effects of various drugs on the GABRA4 receptor, thereby understanding the mechanisms of drugs in treating neurological diseases.

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