Cat.No. : CSC-RI0007 Host Cell: HEK293
| Cat. No. | CSC-RI0007 |
| Description | This cell line is engineered to overexpress human GABRA4 |
| Gene | GABRA4 |
| Gene Species | Homo sapiens (Human) |
| Alias | GABRA4, alpha 4 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line is based on HEK293 cells, with the human GABRA4 (gamma-aminobutyric acid A receptor alpha4 subunit) gene stably transfected into these cells.
A: It is mainly used for studying the role of GABRA4 in neural system functions, especially in neural transmission and emotional regulation.
A: It can be used for screening drugs that affect GABRA4 activity, particularly in the treatment of neurological diseases and mood disorders.
A: The cells should be handled under sterile conditions, with regular monitoring of cell growth and GABRA4 protein expression levels.
A: They should be cultured at 37°C in a 5% CO2 environment, using a medium suitable for the growth of HEK293 cells.
A: It can be used to study the effects of various drugs on the GABRA4 receptor, thereby understanding the mechanisms of drugs in treating neurological diseases.
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