Human FCGRT Stable Cell Line-CHO-K1

Name: Human FCGRT Stable Cell Line-CHO-K1
SKU: CSC-RO0151
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0151

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0151
Description	This cell line stably expresses human Fc fragment of IgG receptor and transporter (FCGRT). The surface expression of the target protein in CHO-FCGRT stable cells was analyzed via flow cytometry.
Target Gene	FCGRT
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ]
Gene Symbol	FCGRT
Synonyms	FCRN; alpha-chain
GeneID	2217
Uni ProtID	P55899
mRNA Refseq	NM_001136019.2
Protein Refseq	NP_001129491.1
Chromosome Location	19q13.3
Function	IgG binding; beta-2-microglobulin binding; receptor activity;
MIM	601437

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the origin of the Human FCGRT Stable Cell Line-CHO, and how was it genetically engineered?

A: The Human FCGRT Stable Cell Line-CHO is derived from Chinese Hamster Ovary (CHO) cells, which are widely used in biotechnology for their ability to produce complex proteins. The cells are genetically engineered to stably express the full-length human FCGRT gene, which encodes the FcRn receptor. This process typically involves the use of a vector containing the FCGRT gene, which is then integrated into the CHO cell genome, allowing for the stable expression of the protein.

Can you describe the genotype and phenotype of the Human FCGRT Stable Cell Line-CHO?

A: The genotype of the Human FCGRT Stable Cell Line-CHO includes the native CHO cell genome, supplemented with the exogenously introduced human FCGRT gene. The phenotype is characterized by the stable expression of the FcRn receptor on the cell surface, which can be confirmed by immunofluorescence or flow cytometry assays.

What are the primary applications of the Human FCGRT Stable Cell Line-CHO in research?

A: The Human FCGRT Stable Cell Line-CHO is used in various research areas, including immunology, pharmacology, and infectious disease studies. It is particularly valuable for studying the interaction of FcRn with immunoglobulins, therapeutic proteins, and viruses, as well as for the development and optimization of monoclonal antibodies and other biologics.

What are the ethical considerations and regulatory requirements associated with the use of Human FCGRT Stable Cell Line-CHO?

A: The use of Human FCGRT Stable Cell Line-CHO must adhere to guidelines set forth by institutional review boards and relevant regulatory bodies. This includes ensuring the cells are sourced ethically, used responsibly, and disposed of in an environmentally sound manner. Researchers must also follow good laboratory practices and comply with any specific regulations regarding genetically modified organisms.

How does the Human FCGRT Stable Cell Line-CHO contribute to the development of therapeutic proteins and vaccines?

A: The Human FCGRT Stable Cell Line-CHO is instrumental in the development of therapeutic proteins and vaccines, particularly those that require FcRn-mediated processes. It can be used to study the stability, half-life, and pharmacokinetics of these molecules, as well as to optimize their design for improved efficacy and safety.

How can the Human FCGRT Stable Cell Line-CHO be used to study the interaction between FcRn and viruses, such as echoviruses?

A: The Human FCGRT Stable Cell Line-CHO can be used to investigate the binding and uncoating mechanisms of viruses like echoviruses, which rely on FcRn for entry into host cells. This can provide insights into viral pathogenesis and inform the development of antiviral strategies.

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