Human CD244 Stable Cell Line-HEK293T

Prostate cancer (PCa) is a leading cause of cancer-related death, with metastasis to bones being the most common and critical complication. The immune system, particularly natural killer (NK) cells, plays a key role in combating tumor spread, and the interaction between immune receptors such as 2B4 (CD244) and its ligand CD48 is vital for NK cell activation. The researchers aimed to investigate the structural requirements for this interaction and how it modulates NK cell activity. They transfected HEK293T cells to stably express CD244 (2B4) and CD48, creating a controllable system to explore the molecular dynamics of this immune modulation. Their results highlighted the importance of both cis and trans interactions between 2B4 and CD48 in regulating NK cell function, and how these interactions influence immune responses against cancer cells.

Figure 1. The researchers examined the impact of CD48 interaction on the surface expression of 2B4 in HEK293T cells by co-culturing cells expressing 2B4 with HEK-CD48 or transfecting them with CD48 vectors. They used flow cytometry to measure 2B4 expression levels, revealing how cis and trans interactions modulate 2B4 expression. (Claus M, et al., 2016)

Creative Biogene's Human CD244 Stable Cell Line - HEK293T can be used for similar studies involving immune receptor signaling, particularly in cancer research. This cell line provides a reliable model for studying the interactions between 2B4 and CD48 and their impact on NK cell activity, offering insights into immune modulation that could lead to novel therapeutic strategies for metastasis.