Cat.No. : CSC-RO0093 Host Cell: HEK293T
| Cat. No. | CSC-RO0093 |
| Description | HEK293T-CD244 cell line is a stably transfected cell line which expresses human CD244 molecule. |
| Gene | CD244 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Prostate cancer (PCa) is a leading cause of cancer-related death, with metastasis to bones being the most common and critical complication. The immune system, particularly natural killer (NK) cells, plays a key role in combating tumor spread, and the interaction between immune receptors such as 2B4 (CD244) and its ligand CD48 is vital for NK cell activation. The researchers aimed to investigate the structural requirements for this interaction and how it modulates NK cell activity. They transfected HEK293T cells to stably express CD244 (2B4) and CD48, creating a controllable system to explore the molecular dynamics of this immune modulation. Their results highlighted the importance of both cis and trans interactions between 2B4 and CD48 in regulating NK cell function, and how these interactions influence immune responses against cancer cells.
Figure 1. The researchers examined the impact of CD48 interaction on the surface expression of 2B4 in HEK293T cells by co-culturing cells expressing 2B4 with HEK-CD48 or transfecting them with CD48 vectors. They used flow cytometry to measure 2B4 expression levels, revealing how cis and trans interactions modulate 2B4 expression. (Claus M, et al., 2016)
Creative Biogene's Human CD244 Stable Cell Line - HEK293T can be used for similar studies involving immune receptor signaling, particularly in cancer research. This cell line provides a reliable model for studying the interactions between 2B4 and CD48 and their impact on NK cell activity, offering insights into immune modulation that could lead to novel therapeutic strategies for metastasis.
A: The Human CD244 Stable Cell Line-HEK293T, which expresses the CD244 receptor, can be used to study the activation and inhibition signals propagated by CD244 interactions within the immune system. Researchers can manipulate the cellular environment to assess how various ligands and coreceptors influence CD244 signaling, leading to a better understanding of immune cell regulation.
A: By providing a consistent and controlled model for CD244 signaling, this cell line can aid in the screening and development of drugs that modulate CD244 activity. Researchers can identify compounds that enhance or inhibit CD244 signaling to develop new treatments for conditions such as autoimmune diseases or cancer, where immune regulation is critical.
A: The cell line can be used to perform binding assays and functional studies to determine the affinity and consequences of the CD244-CD48 interaction. This can help elucidate the molecular basis of how these two proteins interact and influence immune cell behavior.
A: The cell line can be employed to test the efficacy of immunotherapeutic approaches that rely on CD244 signaling, such as blocking the CD244-CD48 interaction to enhance NK cell activity against tumor cells. It can also be used to evaluate the impact of CD244 modulation on T cell responses in the context of adoptive cell transfer therapies.
A: By exposing the cell line to conditions that mimic chronic viral infection or tumor microenvironments, researchers can investigate how CD244 expression and signaling contribute to NK cell exhaustion and the subsequent immune evasion by tumors. This can lead to the identification of novel therapeutic targets to enhance anti-tumor immunity.
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This cell line consistently expresses high levels of CD244, making it particularly useful for assays designed to investigate the function and regulatory mechanisms of this receptor. The Human CD244 Stable Cell Line - HEK293T allows us to reliably assess CD244's role in immune cell activation and inhibition.
The Human CD244 Stable Cell Line - HEK293T is specifically designed for immunological research, enhancing the study of CD244, a receptor involved in immune cell regulation. This cell line provides a consistent model for exploring the interactions and signaling pathways of CD244 in immune responses.
The cell line comes in a ready-to-use format, which reduces the preparatory work required for cell culture and experimental setup. This feature of the Human CD244 Stable Cell Line - HEK293T allows us to quickly initiate experiments, saving time and resources.
Due to its stable expression of CD244, the Human CD244 Stable Cell Line - HEK293T can be effectively used in co-culture systems to study interactions between cells expressing CD244 and immune cells. This versatility is particularly valuable in the study of immune system dynamics and the development of immunotherapies.
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