Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO0278
Host Cell : CHO-K1 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO0278 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Rabbit CD47. |
| Target Gene | CD47 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Applications |
1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Yes, the expression level of CD47 protein in Rabbit CD47 Stable Cell Line - CHO-K1 can be quantitatively analyzed by flow cytometry using specific antibodies.
A: In Rabbit CD47 Stable Cell Line - CHO-K1, the membrane localization of CD47 protein and possible endoplasmic reticulum retention can be detected by immunofluorescence staining and confocal microscopy.
A: Yes, Rabbit CD47 Stable Cell Line - CHO-K1 is suitable for studying the interaction between CD47 and its ligand SIRPα, including binding kinetics and signal transduction mechanisms.
A: The immunosuppressive effect of Rabbit CD47 Stable Cell Line - CHO-K1 can be evaluated by examining its inhibitory effects on immune cells such as macrophages and T cells.
A: When constructing Rabbit CD47 Stable Cell Line - CHO-K1, it's important to choose a vector that ensures stable expression and functionality of CD47 protein, and a resistance gene that provides effective drug selection.
A: Yes, Rabbit CD47 Stable Cell Line - CHO-K1 is suitable for drug screening targeting the CD47 pathway, and can be used to evaluate the effects of candidate drugs on CD47 protein function.
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