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CC-1465

TREX1 Easy KO Kit (Mouse)

For research use only. Not intended for any clinical use.

Cat. No. :   CC-1465

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Product Information

Gene Information

Cat. No. CC-1465
Description A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr Trex1
Gene Name Trex1 three prime repair exonuclease 1 [ Mus musculus ]
Species Mouse
EnsemblID ENSMUSG00000049734
NCBIGeneID 22040
Uni ProtID Q91XB0
Features
  • All-in-One workflow from gene editing to knockout validation for users with no prior experience.
  • Pre-validated sgRNAs and primers for rapid setup.
  • Streamlined experiment handling.
  • CRISPR RNP method ensures precise and efficient gene knockout.
Applications This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions 5–10 reactions per target gene
Kit Components 2–3 chemically synthesized sgRNAs (200pmol each)
3 PCR/Sequencing primers (500pmol each)
LM cell lysate (500µL)
Cas9 protein (12µg)
LM RNP transfection reagent (50µL)
Storage Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.
Gene Name Trex1 three prime repair exonuclease 1 [ Mus musculus ]
Gene Symbol Trex1
Synonyms 1661; AU041952
Gene Description three prime repair exonuclease 1
GeneID 22040
Uni ProtID Q91XB0
mRNA Refseq NM_001012236.1
Protein Refseq NP_001012236.1
Chromosome Location 9 F2; 9
Function 3-5 exonuclease activity; MutLalpha complex binding; MutSalpha complex binding; adenyl deoxyribonucleotide binding; double-stranded DNA binding; exodeoxyribonuclease III activity; exonuclease activity; hydrolase activity; nuclease activity; nucleic acid binding; protein homodimerization activity; single-stranded DNA binding;
Pathway Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem;
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